白芍总苷对动脉粥样硬化大鼠血流动力学和血液流变学的影响

目的]研究白芍总苷(TGP)对动脉粥样硬化大鼠血流动力学和血液流变学的影响并初步探讨其作用机制。方法]用高脂饲料喂养结合腹腔注射维生素D3(VD3)的方法建立大鼠动脉粥样硬化(AS)模型,造模过程分别同步灌胃给予TGP50、100、200 mg/(kg·d)]和辛伐他汀(SV),1.8 mg/(kg·d),共12周,并设模型组和正常对照组。采用苏木精-伊红(HE)染色法观察主动脉形态结构改变并进行病变分级;测定血液流变学指标、血流动力学指标、血清中血脂指标。结果]较模型组,TGP干预组大鼠主动脉病理性形态结构改变呈不同程度改善,其中TGP100、200 mg/(kg·d)]组病变分级显著降低、血液流变学指标全血黏度(高切、中切、低切)、血浆黏度、红细胞压积、血小板黏附率]均显著降低、血流动力学指标心室内压最大上升速率(lv+dp/dtmax)显著升高、心室内压达峰时间(t-dp/dtmax)显著缩短]、血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)含量显著降低,TGP 200 mg/(kg·d)组高密度脂蛋白(HDL-C)显著升高,差异均具有统计学意义(P0.05,P0.01)。结论]TGP可能通过改善血液流变学和血液动力学而对动脉粥样硬化具有一定的保护作用。

Effect and mechanism of total glucosides of paeony on atherosclerosis in rats

Objective] To investigate the effects of total glucosides of paeony (TGP) on hemodynamics and hemorrheology in atherosclerosis rats. Methods] The rat models were made by dirtying with high fat and injecting VD3, and at the same time, which were treated with TGP 50, 100, 200 mg/(kg·d) and simvastatin SV, 1.8 mg/(kg·d)] for 12 weeks, setting normal control group and model group. The histopathological changes of abdominal aorta was observed by HE staining and graded; the indexes of hemorrheology were determined; the level of lv dp/dtmax and t-dp/dtmax were determined; the content of TC, TG, LDL-C, HDL-C in serum were detected. Results] Compared with model group, the histopathological changes of the rats in TGP treated groups were improved. The whole blood viscosity, plasma viscosity, platelet adhesion rate, hematocrit were significantly decreased; the level of lv dp/dtmax was significantly increased and the t-dp/dtmaxwas significantly shortened. The level of TC, TG, LDL-C in serum were significantly decreased while the level of HDL-C was significantly increased, all of the difference above were significant (P<0.05, P<0.01). Conclusion] TGP has protective effects on the rats with atherosclerosis through improve hemorrheology and hemodynamics.