| 鲑鱼降钙素与降钙素基因相关肽融合基因植物表达载体的构建 |
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| 引用本文： | 余 琼,王 超,李荣田,平文祥,孙怡宁,李洪丽.鲑鱼降钙素与降钙素基因相关肽融合基因植物表达载体的构建[J].食品科学,2009,30(5):126-129. |
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| 作者姓名： | 余 琼 王 超 李荣田 平文祥 孙怡宁 李洪丽 |
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| 作者单位： | 黑龙江省高等学校分子生物学重点实验室；黑龙江大学生命科学学院 |
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| 基金项目： | 黑龙江省教育厅2007 年科学技术研究面上项目(11521223) 。 |
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| 摘 要： | 根据基因工程操作方法和植物偏爱密码子原则，设计了鲑鱼降钙素与降钙素基因相关肽融合基因，并在融合基因前端加上AMV RNA4 5＇-UTR 序列和6 × His 标签，在融合基因后边加上一个甘氨酸。通过Sac I 和Sma I双酶切pUC(AMV / CGRP / msCT)获得融合基因，用T4 DNA 连接酶连到植物表达载体pBI121 上，构建重组质粒pBI AMV / CGRP / msCT，再转化到E.coli DH5α中保存。通过DNA 测序验证表达载体构建成功。
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| 关 键 词： | 鲑鱼降钙素 降钙素基因相关肽 基因工程 融合表达 高血压 骨质疏松 |
| 收稿时间： | 2008-03-20 |
Construction of Plant Expression Vector of Salmon Calcitonin (sCT) and Calcitonin Gene-related Peptide (CGRP) Fusion Gene |
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| YU Qiong,WANG Chao,LI Rong-tian,PING Wen-xiang,SUN Yi-ning,LI Hong-li.Construction of Plant Expression Vector of Salmon Calcitonin (sCT) and Calcitonin Gene-related Peptide (CGRP) Fusion Gene[J].Food Science,2009,30(5):126-129. |
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| Authors: | YU Qiong WANG Chao LI Rong-tian PING Wen-xiang SUN Yi-ning LI Hong-li |
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| Affiliation: | (Key Laboratory of Molecular Biology, College of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin
150080, China) |
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| Abstract: | The fusion gene of sCT and CGRP was designed according to the principles of genetic engineering method and the bias codon used by plant. An AMV RNA4 5＇-UTR sequence and a 6×His tag were added before the gene, and the Gly was added at the end of the fusion gene. The fusion gene was obtained from pUC(AMV / CGRP / msCT) by Sac I and Sma I digestion, then cloned into plant expression vector pBI121 by T4 DNA ligase to construct recombinant plasmid pBI AMV / CGRP / msCT, and transformed into E.coli DH5α to be kept. The gene sequencing indicated that the expression vector pBI AMV / CGRP / msCT has been successfully constructed. |
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| Keywords: | salmon calcitonin calcitonin gene-related peptide genetic engineering fusion expression hypertension osteoporosis |
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