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棘孢木霉T-6对烟草促生及对黑胫病和根黑腐病的抗病作用
引用本文:宋玉娟,韩雪莲,张安盛,王玉军,高克祥,梁元存.棘孢木霉T-6对烟草促生及对黑胫病和根黑腐病的抗病作用[J].中国烟草科学,2020,41(1):50-55.
作者姓名:宋玉娟  韩雪莲  张安盛  王玉军  高克祥  梁元存
作者单位:1. 山东农业大学植物保护学院, 山东 泰安 271018;2. 山东省农业科学院植保所, 济南 250100
基金项目:山东省现代农业烟草体系烟草病虫防控岗位(SDAIT-25-3)
摘    要:利用生防菌防治土传病害是植物病害防治中的重要措施,在温室盆栽条件下研究了棘孢木霉T-6对烟草促生和抗病效果。结果表明,棘孢木霉T-6对烟草黑胫病菌、烟草根黑腐病菌的菌丝生长抑制率分别达49.62%、59.23%;温室盆栽条件下,棘孢木霉T-6对黑胫病和根黑腐病的防治效果分别达到74.45%和75.14%。棘孢木霉T-6处理后提高了烟草叶片叶绿素含量、类胡萝卜素含量和超氧化物歧化酶、过氧化物酶、过氧化氢酶的活性,棘孢木霉T-6处理的烟株生长状况明显好于对照。棘孢木霉T-6能够促进烟株生长和提高烟草对黑胫病和根黑腐病的抗性,具有良好的应用潜力。

关 键 词:烟草  棘孢木霉T-6  黑胫病  根黑腐病  生物防治  
收稿时间:2019-06-04

Effects of Trichoderma asperellum T-6 on Growth Promotion,Control of Black Shank and Black Root Rot in Tobacco
SONG Yujuan,HAN Xuelian,ZHANG Ansheng,WANG Yujun,GAO Kexiang,LIANG Yuancun.Effects of Trichoderma asperellum T-6 on Growth Promotion,Control of Black Shank and Black Root Rot in Tobacco[J].Chinese Tobacco Science,2020,41(1):50-55.
Authors:SONG Yujuan  HAN Xuelian  ZHANG Ansheng  WANG Yujun  GAO Kexiang  LIANG Yuancun
Affiliation:1. College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China;2. Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, 250100, China
Abstract:Using Trichoderma spp.to control plant soil-borne diseases is a promising field in biological control.In order to investigate the effect of T.asperellum T-6 on tobacco growth and resistance to soil-borne fungi,spore suspensions of T.asperellum T-6 were inoculated into soil,and then pot experiments were conducted in greenhouse.The results showed that hyphal growth of Phytophthora parasitica and Thielaviopsis basicola was inhibited by T.asperellum T-6,and the inhibited rates were 49.62%and 59.23%,respectively.Control efficiency of T.asperellum T-6 on black shank and black root rot reached 74.45%and 75.14%.After T.asperellum T-6 pretreatment,the content of chlorophyll and carotenoids and the activities of three resistance-related enzymes in tobacco leaves were increased,and the growth of tobacco treated with T.asperellum T-6 were better than control.Taken together,the results from this study indicated that T.asperellum T-6 could control tobacco black shank and black root rot effectively,and promote tobacco growth.
Keywords:tobacco  Trichoderma asperellum  black shank  black root rot  biological control
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