Effect of substrate and fermentation conditions on pectinase and cellulase production by <Emphasis Type="Italic">Aspergillus niger</Emphasis> NCIM 548 in submerged (SmF) and solid state fermentation (SSF) |
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Authors: | Sanjay Kumar H K Sharma B C Sarkar |
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Affiliation: | (1) Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India |
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Abstract: | The present study deals with the optimization of substrate and fermentation conditions for the production of both pectinase
and cellulase by Aspergillus niger NCIM 548 under same fermentation conditions in submerged fermentation (SmF) and solid state fementation (SSF) using a central
composite face centered design of response surface methodology (RSM). As per statistical design, the optimum conditions for
maximum production of pectinase (1.64 U/mL in SmF and 179.83 U/g in SSF) and cellulase (0.36 U/mL in SmF and 10.81 U/g in
SSF) were, time 126 h, pH 4.6, and carbon source concentration 65 g/L in SmF and were time 156 h, pH 4.80, and moisture content
65% in SSF. The response surface modeling was applied effectively to optimize the production of both pectinase and cellulase
by A. niger under same fermentation conditions to make the process cost-effective in both submerged and solid state fermentation using
agro industrial wastes as substrate. |
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Keywords: | |
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