白介素-17对大鼠心肌梗死后心室重塑的影响

目的探讨大鼠心肌梗死(MI)后白介素-17(IL-17)的表达在MI后心室重塑发生中的作用。方法采用液氮冷冻法建立大鼠MI 4周和8周模型。将MI大鼠随机分为MI对照组(C组)、小鼠同型IgG组(G组)和IL-17抗体组(A组)。每组又随机分为4周和8周亚组,每个亚组约8～10只。另设假手术组8只(S组)。共54只。S组和C组分别腹腔注射磷酸盐缓冲液(PBS)(0.8mL PBS溶液,并注入0.2mL空气促使液体分散到腹腔内),G组、A组分别注射小鼠同型IgG1+PBS溶液(100μgIgG1溶于0.8mL PBS,同样注入0.2mL空气)、小鼠抗人IL-17单克隆抗体+PBS溶液(100μg IL-17抗体溶于0.8mL PBS溶液,同样注入0.2mL空气)。每隔1天用药1次,连续用药5次。应用超声心动图及病理学方法测定胶原的表达以评价大鼠心功能变化和心室重塑的过程,同时用Western blot检测IL-17蛋白表达水平。结果超声心动图检测显示大鼠心室重塑模型构建成功。与S组比较,MI组大鼠心肌组织IL-17表达显著增加(P<0.05),与MI组比较,A组IL-17表达显著减少(P<0.05),超声心动图各相关指标明显改善(P<0.05),Ⅰ/Ⅲ胶原比例较之显著降低(P<0.05)。而G组与C组相比无明显差异(P>0.05)。结论 MI后大鼠心肌组织IL-17表达明显增加,IL-17表达可能与MI后心室重塑的发生密切相关。

The effect of IL-17 expression on ventricular remodeling after myocardial infarction in rats

Objective To investigate the effect of IL-17 expression on ventricular remodeling with myocardial infarction(MI) in rats.Methods Liquid nitrogen cryoinjury method was used to establish myocardial infarction model.The MI models were randomly divided into 3 groups,MI control group(C group),IgG group(G group) and antibody group(A group).Then each group was randomly divided into 2 sub-groups:4 week and 8 week groups,each sub-group of 8 to 10.Eight rats of sham group underwent sham operation,being killed after 8 w(S group).The first day after operation,PBS solution were intraperitoneally injected to sham group and C group,mouse isotype IgG1 and PBS to G group,monoclonal human IL-17 antibody and PBS to A group,5 times every two days.Cardiac function and ventricular remodeling were evaluated by echocardiography and morphological examination.The protein expression of I,III collagen was assessed by the method of immunohistochemical stain.The protein expression of interleukin-17 was examined by Western blot.Results Echocardiogram demonstrated that rat models were successfully made.Compared to sham group,expression of IL-17 in myocardium in MI control group,IgG group and antibody group increased obviously at 4 weeks and 8 weeks after operation(P<0.05)and ventricular remodeling related parameters LVEDD,LVESD,EDV,ESV,LV/BW increased(P<0.05),LVEF,LVFS decreased(P<0.05);Compared to MI control group and IgG group,expression of IL-17 in antibody group was lower(P<0.05),and the parameters improved(P<0.05).Ⅰ/Ⅲ collagen ratio were also significantly decreased in the 4 w,8 w sub-groups of Antibody group(P<0.05).While,obvious differences were not found between MI control group and IgG group.Conclusion IL-17 expression was significantly increased after MI.There is relationship between a large number of IL-17 expression and ventricular remodeling in rats with MI.