| 红茶菌抗菌蛋白提取与纯化 |
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| 引用本文： | 谢俊杰,佘世望,赵利,熊勇华,钟青萍,龙钢.红茶菌抗菌蛋白提取与纯化[J].氨基酸和生物资源,2000,22(1):16-19. |
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| 作者姓名： | 谢俊杰 佘世望 赵利 熊勇华 钟青萍 龙钢 |
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| 作者单位： | 中德联合研究院,江西南昌,330047 |
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| 摘 要： | 红茶菌液经过滤、离心、减压浓缩 ,丙酮加盐沉淀 ,Sephadex G-50柱层析后 ,得到两个分子量部分 ,其中小分子量部分有抗菌活性 ,采用尿素— SDS—PAGE测定抗菌蛋白分子量未获成功
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| 关 键 词： | 红茶菌 抗菌蛋白 提取 纯化 |
Purification of the antibacterial protein from tea fungus culture |
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| Xie Junjie,She Shiwang,Zhao Li,Xiong Yonghau,Zhaong Qingping,Long Gang.Purification of the antibacterial protein from tea fungus culture[J].Amino Acids & Biotic Resources,2000,22(1):16-19. |
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| Authors: | Xie Junjie She Shiwang Zhao Li Xiong Yonghau Zhaong Qingping Long Gang |
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| Abstract: | By filtration, centrifugation, vacuum concentration, extraction with acetone and inorganic salts, and filtration chromatography through Sephadex G 50, two proteins with different MW were obtained from the tea fungus culture. The protein with low MW demonstrated antibacterial activity. Unfortunately, the determination of the molecular weight of the protein with urea -SDS-PAGE was failed. |
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| Keywords: | Tea fungus Antibacterial protein Isolation Purification |
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