云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用

目的建立云南省玉溪市甲型副伤寒沙门菌（SPA）分离株脉冲场凝胶电泳（PFGE）数据库，分析玉溪市各分子型别SPA的流行和分布情况，为本市SPA的监测和疫情的应急处理提供科学依据。方法采用Spe Ⅰ 和 Xba Ⅰ消化细菌染色体，对来自玉溪市6县1区194株SPA分离株进行PFGE分型和聚类分析。结果194株SPA中， Xba Ⅰ带型共有7种，分别为Xba Ⅰ 01、Xba Ⅰ 02、Xba Ⅰ 06、Xba Ⅰ 07、Xba Ⅰ 09、Xba Ⅰ 10、Xba Ⅰ 19，其中Xba Ⅰ 01为优势带型，占93.81％（182/194）；Spe Ⅰ带型共有10种，分别为Spe Ⅰ01~10，其中Spe Ⅰ 01、Spe Ⅰ 02为优势带型，各占46.39%（90/194） 和 43.30%（84/194）。比较分析Xba Ⅰ带型和Spe Ⅰ 带型，Spe Ⅰ 酶切比Xba Ⅰ酶切具有更多的带型，主要是Xba Ⅰ 01带型和Xba Ⅰ 02带型可以分为更多的Spe Ⅰ带型，Spe Ⅰ 01和Spe Ⅰ 02带型均来自于Xba Ⅰ 01带型。但是，也有不同的Xba Ⅰ带型归属于相同的Spe Ⅰ带型。结论建立了玉溪市SPA PFGE数据库，Spe Ⅰ酶切数据库与Xba Ⅰ酶切数据库具有较好的一致性。Spe Ⅰ 01和Spe Ⅰ 02或Xba Ⅰ 01是玉溪流行的主要克隆，同时也不容忽视其他分子型别的危害。

Establishment and application of PFGE database on Salmonella enterica serotype Paratyphi A in Yuxi,Yunnan

Objective To establish the PFGE database on Salmonella enterica serotype Paratyphi A （SPA） in Yuxi and to identify the distribution of SPA of varying subtypes in Yuxi, providing scientific basis for surveillance and emergency response of SPA in the city. Methods The chromosomes of 194 SPA strains isolated from seven counties in Yuxi were digested with restriction endonuclease Xba I and Spe I. PFGE typing and cluster analysis was employed to determine the distribution of SPA. Results There were seven XbaI subtypes out of 194 SPA strains, including Xba I01, Xba I02, Xba I06, Xba I07, Xba I09, Xba I10 and Xba I19, of which Xba 101 predominated with the proportion of 93.81% （182/194）. There were ten Spe I subtypes （ Spe I01 - 10） out of 194 strains, Spe I01 and Spe 102 being prevalent with the proportion of 46.39% （ 90/194 ） and 43.30% （ 84/194 ）, respectively. Comparison between Xba I and Spe I band patterns suggested greater amount of band patterns of Xba I digestion than that of Spe I digestion, which was due to more Spe I patterns derived from Xba I01 and Xba I02 in contrast with Spe I01 and Spe I02 patterns originated solely from Xba I01. However, different Xba I patterns were found to generate the same Spe I band. Conclusion The PFGE database featuring highly-consistent SpeI and Xba I digestion datasets was established. Although SpeI01 and SpeI02 （or Xbal01 ） constituted the major prevalent clones, other detrmental subtypes must not be ignored in Yuxi.