板蓝根多肽对流感病毒感染小鼠抗病毒作用的研究

目的:研究板蓝根多肽对流感病毒感染小鼠的抗病毒作用。方法:以4倍半数致死量的流感病毒H1N1(A/PR8/34)液滴鼻感染以复制小鼠流感病毒感染模型。小鼠均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、利巴韦林(腹腔注射,40 mg/kg)组与板蓝根多肽高、中、低剂量(灌胃给药,200、100、50 mg/kg)组,连续给药7 d。测定小鼠死亡率、生存时间、肺指数、病毒滴度与T、B淋巴细胞增殖情况。结果:与正常对照组比较,模型组小鼠死亡率升高,生存时间缩短,肺指数升高,T、B淋巴细胞减少,差异有统计学意义(P<0.01)。与模型组比较,板蓝根多肽高、中剂量组小鼠死亡率降低,生存时间延长,感染第5天肺指数降低,差异有统计学意义(P<0.01或P<0.05);板蓝根多肽高剂量组小鼠感染第3天病毒滴度降低,差异有统计学意义(P<0.05);板蓝根多肽高、中、低剂量组小鼠T、B淋巴细胞增加,差异有统计学意义(P<0.01或P<0.05)。结论:板蓝根多肽具有明显的抗病毒和促进免疫的作用。

Study on Anti-virus Effect of Peptides from Isatis indigotica on the Mice Infected by Influenza Virus

OBJECTIVE： To study the anti-virus effect of peptides from Isatis indigotica on the mice infected with influenza vi- rus. METHODS ： Mice models of influenza virus infection were induced by nasal drops of 4LD50 influenza virus H1N1 （A/PR8/34）. Mice were divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline） , ribavirin group （i.p., 40 mg/kg, consecutive 7 days） and I. indigotica peptides high-dose, medium-dose and low-dose groups （i.g., 200, 100, 50 mg/kg, consecutive 14 days）. The mortality, survival time, lung index, virus titer, the proliferation of T and B lymphocyte in mice were determined. RESULTS： Compared with normal control group, the mortality, lung index of mice in model group were increased significantly, while survival time, the proliferation of T and B lymphocyte was decreased significantly; there was statistical significance （P〈0.01）. Compared with model group, the mortality, survival time and lung index on 5th day were decreased significantly in L indigotica peptides high-dose and medium-dose groups; there was statistical significance （P〈 0.01 or P〈0.05） ; virus titer of/. indigotica peptides high-dose was decreased significantly on 3rd day; there was statistical signiflcance（P〈0.05）;T and B lymphocyte of I. indigotica peptides high-dose, medium-dose and low-dose groups were increased significantly; there was statistical significance （P〈 0.01 or P〈 0.05）. CONCLUSIONS：I. indigotica peptides can obviously inhibit influenza virus in vivo and improve immune function.