| HPLC法测定氯霉素阴道片中氯霉素的含量 |
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| 引用本文： | 时正媛,曾蔚欣,孙路路.HPLC法测定氯霉素阴道片中氯霉素的含量[J].中国药师,2014(10):1788-1789. |
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| 作者姓名： | 时正媛 曾蔚欣 孙路路 |
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| 作者单位： | 首都医科大学附属北京世纪坛医院药剂科 北京 100038;首都医科大学附属北京世纪坛医院药剂科 北京 100038;首都医科大学附属北京世纪坛医院药剂科 北京 100038 |
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| 基金项目： | 首都卫生发展科研专项-重点攻关项目（编号：首发2011-2008-01） |
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| 摘 要： | 目的：建立测定氯霉素阴道片中氯霉素含量的 HPLC法。方法：色谱柱为 Agilent ZORBAX SB-C18柱（150 mm ×4.6 mm,5μm）,流动相为含0.1%庚烷磺酸钠的溶液-甲醇（68∶32）,流速1 ml·min^-1,检测波长277 nm,柱温35℃,进样量10μl。结果：氯霉素浓度在25.6~512.0μg·ml^-1范围内与峰面积线性关系良好,r=0.9999,平均回收率为99.4%,RSD为0.8%（n=9）。结论：该法简便、准确、重复性好,适用于测定氯霉素阴道片中氯霉素的含量。
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| 关 键 词： | 高效液相色谱法 氯霉素 阴道片 含量测定 |
| 收稿时间： | 2014/3/25 0:00:00 |
| 修稿时间： | 2014/6/19 0:00:00 |
Determination of Chloramphenicol Vaginal Tablets by HPLC |
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| Shi Zhengyuan,Zeng Weixin and Sun Lulu.Determination of Chloramphenicol Vaginal Tablets by HPLC[J].China Pharmacist,2014(10):1788-1789. |
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| Authors: | Shi Zhengyuan Zeng Weixin and Sun Lulu |
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| Affiliation: | ( Department of Pharmacy, Beijing Shijitan Hospital Affiliated to Capital Medical University, Bei- jing 100038 ,China) |
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| Abstract: | Objective：To establish an HPLC method for the determination of chloramphenicol in chloramphenicol vaginal tablets. Methods：An Agilent ZORBAX SB-C18 column （150 mm × 4. 6 mm,5 μm）was used. The mobile phase was composed of 0. 1% sodi-um 1-heptanesulfonate solution-methanol （68∶32）,the flow rate was 1. 0 ml·min^-1, the UV detection wavelength was 277 nm,the column temperature was 35℃,and the injection volume was 10 μl. Results：The linear range of chloramphenicol was 25. 6-512. 0 μg ·ml^-1（r=0. 999 9）. The mean recovery was 99. 4%, and RSD was 0. 8%（n=9）. Conclusion：The method is simple,accurate and reproducible,and can be used in the determination of chloramphenicol vaginal tablets. |
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| Keywords: | HPLC Chloramphenicol Vaginal tablets Determination |
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