| 郫县豆瓣原料蚕豆中黄曲霉毒素B1的提取 |
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| 引用本文： | 靳学敏,车振明,汪颖.郫县豆瓣原料蚕豆中黄曲霉毒素B1的提取[J].中国调味品,2011,36(3). |
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| 作者姓名： | 靳学敏 车振明 汪颖 |
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| 作者单位： | 西华大学,生物工程学院,成都,610039 |
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| 基金项目： | 西华大学食品科学重点学科资助 |
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| 摘 要： | 郫县豆瓣中所用蚕豆是产自四川省和云南省的优质干蚕豆,经筛选、浸泡、接种、制曲等一系列加工后形成调味豆瓣酱。采用酶联免疫吸附法(ELISA),根据Box-Behnken Design软件的设计原理,检测原料蚕豆中黄曲霉毒素B1。探讨了甲醇浓度、料液比、超声波提取时间等因素对样品中黄曲霉毒素B1提取的影响,以确定检测原料蚕豆中黄曲霉毒素B1含量的最优方案。以黄曲霉毒素B1含量为响应值,利用响应面分析法对提取参数进行优化。结果表明:甲醇浓度43.41%,料液比1∶5.35,超声波提取时间39.89 min是检测原料蚕豆中黄曲霉毒素B1含量的最优条件,以该优化条件检测原料蚕豆中黄曲霉毒素B1其值达到最大,为1.50μg/kg。
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| 关 键 词： | 酶联免疫吸附法 黄曲霉毒素B1 响应面法 |
Detection of aflatoxin B1 from raw material bean of Pixian soybean paste |
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| JIN Xue-min,CHE Zhen-ming,WANG Ying.Detection of aflatoxin B1 from raw material bean of Pixian soybean paste[J].China Condiment,2011,36(3). |
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| Authors: | JIN Xue-min CHE Zhen-ming WANG Ying |
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| Affiliation: | JIN Xue-min,CHE Zhen-ming,WANG Ying(Biological Engineering Institute of Xihua University,Chengdu 610039,China) |
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| Abstract: | Bean used in Pixian soybean paste are produced in Sichuan Province and Yunnan Province,thick broad-bean sauce is produced by screening,dipping,vaccination and a series of process.In this paper,according to the Box-Behnken Desigh software,we used the enzyme-linked immuneosorbent assay(ELISA) to test the valve of aflatoxin B1 in raw material of soybean.Taking over the influence about methanol concentration,ratio of material to solvent and ultrasonic time on the extraction of aflatoxin B1 in the samples to det... |
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| Keywords: | enzyme-linked immunosorbent assay aflatoxin B1 response surface method |
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