| 放射性核素89Sr诱发K562癌细胞凋亡的放射毒理效应的分子机理研究 |
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| 引用本文： | 张军宁,洪承皎,朱寿彭.放射性核素89Sr诱发K562癌细胞凋亡的放射毒理效应的分子机理研究[J].辐射研究与辐射工艺学报,2003,21(1):77-80. |
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| 作者姓名： | 张军宁 洪承皎 朱寿彭 |
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| 作者单位： | 苏州大学附属第一医院,苏州,215006 |
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| 基金项目： | 苏州大学校科研和教改项目,, |
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| 摘 要： | 采用分子病理和定量病理技术研究放射性核素89Sr诱导的K562癌细胞凋亡、剂量效应关系和重要相关基因bcl-2和bax在其中的表达.结果表明,K562细胞经89Sr作用达6h和24h,提取DNA,琼脂糖凝胶电泳图谱上出现典型的"阶梯状"区带,伴有"拖尾",而对照组未见"阶梯状"区带;荧光双标记流式细胞仪法定量检测见89Sr作用6、9、12、24和48h后,K562细胞凋亡率、K562细胞坏死率均较对照组有明显的升高(p<0.01).免疫组织化学染色显示,对照组K562细胞中bcl-2 、bax均有表达,bcl-2表达阳性细胞率82.8%,bax表达阳性细胞率44.4%, bcl-2/bax比值1.86,89Sr作用24h后的K562细胞中, bcl-2表达阳性细胞率31.4%,bax表达阳性细胞率38.2%, bcl-2/bax比值0.82,与对照组相比,差异具有显著性(p<0.05);结果显示,放射性核素89Sr照射K562细胞后,细胞凋亡与细胞坏死并存,且89Sr诱导K562细胞的凋亡可能是通过bcl-2 表达蛋白降低,bcl-2/ bax比值下降而调控的.
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| 关 键 词： | 放射性核素 K562癌细胞 放射毒理效应 分子机理 ^89Sr K562细胞 肿瘤 细胞凋亡 临床治疗 |
| 修稿时间： | 2001年11月13 |
THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K562 CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89 |
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| ZHANG Junning HONG Chengjiao ZHU Shoupeng.THE CHARACTERISTICS AND MECHANISM OF APOPTOSIS IN K562 CELLS INDUCED BY RADIONUCLIDES STRONTIUM-89[J].Journal of Radiation Research and Radiation Processing,2003,21(1):77-80. |
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| Authors: | ZHANG Junning HONG Chengjiao ZHU Shoupeng |
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| Abstract: | In this paper, apoptosis and expressions of bcl-2 and bax protein in K562 cells exposed to different doses of strontium-89 were studied. Six hours after the irradiation, characteristics of apoptosis and necrosis appeared in the K562 cells. The apoptosis and necrosis in the cells irradiated for 6, 9, 12, 24 and 48h were significantly higher than the control. The expression of bcl-2 decreased after the irradiation, whereas bax had no obvious changes. The results suggested that the apoptosis of K562 cells induced by exposure to 89Sr might be regulated by reduced expression of bcl-2 and bcl-2/bax ratio. |
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| Keywords: | Strontium-89 K_(562) cells Apoptosis Bcl-2 Bax |
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