脂质体前列腺素E对大鼠内毒素性急性肺损伤的影响

目的探讨脂质体前列腺素E对内毒素性急性肺损伤血气分析和肺组织改变的影响。方法将SD大鼠随机分为生理盐水对照组、内毒素脂多糖（LPS）急性肺损伤模型组及脂质体前列腺素E干预组。抽取腹主动脉和下腔静脉血液监测血气分析,计算灌支气管肺泡灌洗液（BALF）中白细胞数量及测定总蛋白总量,取肺组织进行病理学观察。结果脂质体前列腺素E显著减少LPS所致BALF中白细胞的数量及总蛋白的浓度（P〈0.01）,降低LPS组大鼠动-静脉血PCO2、PH差值和静脉血乳酸浓度（P〈0.05）,减轻LPS所致的肺组织出血及炎性细胞浸润。结论脂质体前列腺素E可减轻LPS所致的急性肺损伤改变。

The effection of lipo-PGEI on LPS-induced acute lung injury in animals

Aim To ivestigate the role of Lipo-prostaglandin E1（lipo-PGE1） in regulation of lipopolysaccharide（LPS）-induced lung injury in animals.Methods Thirty-two SD rats were averagely and randomly divided into saline control group（n=8 each）,LPS（n=12 each） and LPS＋Lipo-PGE1 group（n=12 each）.LPS model were intravenously injected with LPS（5mg/kg）.The rats in PGE1 group were intravenously injected with PGE1（10μg/kg） ten minutes after LPS were injected into them.The concentrations of lactate and the numbers of white blood cells（WBC） were also analyzed;The pathological changes in lung tissues also in bronchoalveolar lavage fluids（BALF） were detected;The arterial blood gas and venous blood gas ere observed.Results In comparison with those in control group,the white blood cells（799.5±217.32 vs 219.17±102.17,P0.01） and total proteins（0.71±0.083 vs 0.2±0.059,P0.01） in bronchoalveolar lavage fluids（BALF） increased obviously.Compared with the LPS groups,the white blood cells（292.73±42.9 vs 799.5±217.32,P0.01） and total proteins（0.543±0.064 vs 0.71±0.083,P0.01）;In comparison with those in control group,the blood lactate concentrations and blood gas were obviously changed.The values of A-VpH（0.10±0.027 vs 0.0720±0.043,P0.05） and A-VpCO2（26.41±10.49 vs 22.34±9.72,P0.05） and lactate concentration（4.36±2.78 vs 2.81±0.79,P0.05） were increased obviously.Compared with the LPS groups,The values of A-VpH（0.078±0.031 vs 0.10±0.027,P0.05） and A-VpCO2（21.92±5.84 vs 26.41±10.49,P0.05） and lactate concentration（3.86±1.39 vs,4.36±2.78,P0.05） were significantly decreased in the PGE1 groups;In LPS group,the progressively pathological damages in the lung tissues were clearly observed.Conclusion PGE1 plays an essential role in regulation of LPS-induced acute lung injury.The WBC counts and TP concentration in BALF were reduced and the pathological changes in lung tissues relieved.