Bioleaching of sphalerite by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans cultured in 9K medium modified with pyrrhotite |
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Authors: | Song Chen Guan-zhou Qiu Wen-qing Qin and Zhuo-yue Lan |
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Affiliation: | (1) School of Resource Processing and Bioengineering, Central South University, Changsha, 410083, China |
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Abstract: | Elective culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in 9K medium modified with pyrrhotite was studied. Bioleaching of flotation concentrate of sphalerite by the selected bacteria
was carried out. The results show that the microorganisms cultured by pyrrhotite are a mixture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, of which the capability to oxidize ferrous to ferric irons is enhanced by the high mass ratio of Fe to S in pyrrhotite.
Three pyrrhotite samples were separated into various parts with corresponding S/Fe ratios by magnetic separation and were
used to culture the elective bacteria as the substrate. The association of the cultures could provide a more rapid and complete
oxidation of sphalerite than that of bacteria cultivated by conventional methods.
Foundation item: Project(50621063) supported by the National Natural Science Foundation of China; Project(2004CD619205) supported by the Major
State Basic Research Development Program of China |
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Keywords: | bioleaching pyrrhotite elective culture sphalerite |
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