汕头市城镇妇女人乳头状瘤病毒核酸分型流行病学调查

目的 了解汕头市城镇健康妇女人乳头状瘤病毒(HPV)感染情况和核酸分型流行病学调查.方法 采用导流杂交(FH)低密度基因芯片技术,对3 399例健康妇女宫颈脱落细胞进行HPV基因分型检测,并对高危型HPV感染阳性人群进行液基薄层细胞学(TCT)检查.结果 3399例妇女HPV阳性315例,总阳性检出率为9.27%(315/3 399),其中高危型HPV感染率为8.15%(277/3 399)、低危型HPV感染率为2.62%(89/3 399),本地区健康妇女HPV亚型感染最常见依次分别为HPV52、HPV58、HPV16,该人群未检出HPV43.在315例HPV阳性中,以单一HPV亚型感染为主,占86.3%(272/315),并以高危型HPV为主,排在前3位依次为HPV52(21.6%,68/315),HPV16(11.1%,35/315),HPV58(11.1%,35/315).各年龄段HPV感染率随着年龄的增长呈逐渐上升趋势;感染高危型HPV人群宫颈上皮内瘤变发生率为5.4%.结论 健康妇女中有相当比例的女性感染HPV,并以单一高危型为主,HPV亚型感染具有独特的地方特征;结合TCT检查,可早期发现宫颈病变及癌变发生、降低宫颈癌的发生率.

Epidemiological investigation on human papillomavirus nucleic acid genotyping in townswomen of Shantou city

Objective To understand woman human papilloma virus(HPV) infection situations and investigate nucleic acid genotyping among townswomen in Shantou city.Methods By using FH(Flow-through Hybridization) low density gene chip technology,3 399 samples of woman′ cervix uteric cells from ordinary females were checked for HPV genotype.And the high-risk HPV group was tested by TCT(Thinprep cytologic test).Results Of the 3 399 women investigated,315 cases were positive with HPV.The positive rate was 9.27%(315 to 3 399).Among these positive cases,the infection rate of high risk HPV was 8.15%(277 to 3 399),and that of the low risk HPV was 2.62%(89 to 3 399).The most common subtypes of HPV in this area were HPV52,HPV58 and HPV16 in decreasing order,no HPV43 was found.Of the 315 positive samples,pure subtype of HPV was the overwhelming majority,accounted for 86.3%(272 to 315),the most common type was the high risk HPV.HPV52(21.6%,68 to 315),HPV16(11.1%,35 to 315)and HPV58(11.1%,35 to 315)were the three types ranked at the top.The HPV infection rate increased with age.The incidence rate of cervical epithelial neoplasia was 5.4% in high risk HPV infected group.Conclusion A significant amount of women were infected with HPV,mainly with the single high risk type.The infection of HPV subtypes has special regional feature.Helped by TCT,cervical lesion and cervical cancer can be discovered early,which could reduce the incidence of cervical cancer.