乙型肝炎病毒核酸荧光定量及血清标志物与肝功能检测的临床意义

目的探讨乙型肝炎病毒(HBV)感染者 HBV DNA 检出情况及其与血清病毒标志物及肝功能在临床应用的意义.方法采用实时荧光定量聚合酶链反应(FQ‐PCR)检测296例不同血清学类型的 HBV 感染者血清中的 HBV DNA ,同时检测 HBV 血清标志物与肝功能.结果99例 HBeAg (+)标本中 HBV DNA 阳性率为100%,平均 HBV DNA 含量为1.21×107 copy/mL ,其 HBV DNA 检出率和含量与 HBeAg(-)模式的检出情况比较差异有统计学意义(P<0.01);HBsAg (+)与 HBsAg (-)模式比较,HBV DNA 检测结果差异有统计学意义(P<0.01);肝功能正常组与肝功能异常组 HBV DNA 检出率比较差异有统计学意义(P<0.01).58例 HBsAg (-)且肝功能又正常的标本中检出了4例 HBV DNA 阳性.结论实时 FQ‐PCR 定量检测 HBV DNA 与 HBV 血清标记物及肝功能指标联合应用可使诊断更准确,治疗更合理.

The clinical significance of HBV-DNA combine with liver function and HBV serm makers

Objective To investigate the positive rat of HBV DNA, and the value of detection with HBV DNA, sera viral marker,five index of hepatitis B and hepatic function in clinical. Methods 296 cases with HBV were detected about HBV-DNA, sera viral markers and hepatic function by real-time fluorescence quantitative PCR （FQ PCR）. Results 99 cases were diagnosed as HBeAg（T）carrying HBV-DNA（1.21 X 107 copy/mL）,and the positive rat of HBV-DNA was 100~. 4 of 58 cases with HBsAg（- ） were found to be positive HBV-DNA infection. The pos itive rat of HBV-DNA in the patients with HBeAg（- ） was significantly lower than t patients with HBeAg（ ＋ ）（P~ 0.01）. The positive rat of HBV-DNA in the patients with normal hepatic function was significantly lower than pa- tients with abnormal hepatic function（P%0.01）. Conclusion FQ-PCR is a valuable tool for the detection of HBV in the clinical,and it can be used to detect the status of HBV-DNA in a short time. It is better to diagnose type B hepatitis by HBV-DNA combined other detections,such as sera viral marker,five index of hepatitis B and hepatic function at the same time.