| 小鼠胚胎干细胞的分离培养与鉴定 |
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| 引用本文： | 沈岳飞,罗杰峰,莫雪安,龙桂芳.小鼠胚胎干细胞的分离培养与鉴定[J].广西医科大学学报,2006,23(1):43-45. |
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| 作者姓名： | 沈岳飞 罗杰峰 莫雪安 龙桂芳 |
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| 作者单位： | 广西医科大学第一附属医院神经内科,南宁,530021 |
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| 基金项目： | 广西留学回国人员科学基金 |
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| 摘 要： | 目的:培养、分离和鉴定C57BL/ 6小鼠胚胎干细胞(embryonic stem cell ,ESC).方法:收集小鼠3.5 d 的囊胚进行培养,用小鼠胚胎成纤维细胞作为饲养层,形成的ESC样集落,经两次亚克隆法分离培养成ESC;进行相差显微镜观察,采用阶段特异性胚胎抗原-1 (stage-spesific embryonic antigen,SSEA-1)对培养的ESC进行免疫组化染色. 结果:获得了稳定的ESC集落,传至第12代.ESC呈集落样生长,SSEA-1免疫组化染色强阳性.结论:两次亚克隆法获得的细胞具有ESC主要的生物学特性.
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| 关 键 词： | 小鼠 胚胎干细胞 |
| 收稿时间： | 2005-06-17 |
| 修稿时间： | 2005年6月17日 |
ISOLATION, CULTURE AND IDENTIFICATION OF MURINE EMBRYONIC STEM CELLS OF C57BL/ 6 MICE |
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| Shen Yuefei, Luo Jiefeng, Mo Xuean,et al..ISOLATION, CULTURE AND IDENTIFICATION OF MURINE EMBRYONIC STEM CELLS OF C57BL/ 6 MICE[J].Journal of Guangxi Medical University,2006,23(1):43-45. |
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| Authors: | Shen Yuefei Luo Jiefeng Mo Xuean |
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| Affiliation: | Department of Neurology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021 China |
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| Abstract: | Objective:To establish the embryonic stem cell (ESC) lines from C57BL/ 6 species mouse by using the fibroblast cell feeder layers. Methods:The blastocysts of 3.5 d from C57BL/ 6 species mouse were collected and cultured on the fibroblast cell feeder layers. The ES cell-like colonies were sub-clonally cultured twice and the cells were observed with phase contrast microscope and identified by SSEA-1 immunostaining. Result:The stable ESC clones were obtained and were passaged to the 12th generation. ESC was colony-like,and SSEA-1 immunostaining is positive.Conclusion:The cells obtained through twice sub-clonally culturing still maintain the main characteristics of ESC. |
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| Keywords: | mouse embryonic stem cells |
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