| 阿魏侧耳多糖酶水解制备工艺研究 |
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| 引用本文： | 王莹,李国玉,黄健,魏秀岩,马跃平,高建波,王金辉.阿魏侧耳多糖酶水解制备工艺研究[J].中药研究与信息,2011(7):42-44,51. |
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| 作者姓名： | 王莹 李国玉 黄健 魏秀岩 马跃平 高建波 王金辉 |
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| 作者单位： | [1]石河子大学药学院,新疆石河子832002 [2]沈阳药科大学中药学院,辽宁沈阳110016 [3]教育部省部共建新疆特种植物药资源重点实验室,新疆石河子832002 |
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| 基金项目： | 国家863项目（2008AAl02323） |
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| 摘 要： | 目的：采用蛋白酶水解法提取制备阿魏侧耳多糖。方法：采用硫酸一苯酚显色测定多糖的含量，采用UV法测定蛋白质的含量。研究不同蛋白酶在不同条件下对阿魏侧耳多糖提取率和除蛋白效果的影响。结果：最佳酶底比为8mg·g^-1，温度60℃，最适pH值为6．0，最佳酶解时间为90min。结论：采用蛋白酶水解法可以制得纯度很高的阿魏侧耳多糖。
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| 关 键 词： | 蛋白酶水解法 阿魏侧耳多糖 提取制备工艺 |
Study on Extraction of Polysaccharide of Pleurotus ferulae |
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| WANG Ying,LI Guo-yu,HUANG Jian,WEI Xiu-yan,MA Yue-ping,GAO Jian-bo,WANG Jin-hui.Study on Extraction of Polysaccharide of Pleurotus ferulae[J].Research & Information on Traditional Chinese Medicine,2011(7):42-44,51. |
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| Authors: | WANG Ying LI Guo-yu HUANG Jian WEI Xiu-yan MA Yue-ping GAO Jian-bo WANG Jin-hui |
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| Affiliation: | 1. School of Pharmacy, Shihezi University, Shihezi 832002, China ; 2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. Key Laboratory of Phytomedicine Resources ＆ Modernization of TCM , Shihezi 832002, China) |
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| Abstract: | Objective： Enzymatic hydrolysis processes of polysaccharides from Pleurotusfendae were investigated.Methods： Enzymatic hydrolysis processes were developed by from Pleurotus ferulae by using UV spectrophotometry method. determination the concentration of the polysaceharides Results： The enzyme concentration （ 8 mg· g ^- 1 ）, time （60 ℃ ）, pH 5.0, and temperature （ 120 min ） of hydrolysis processes, was developed. Conclusion： Enzymatic hydrolysis is powerful for extracting the polysaeeharide from Pleurotus ferulae. |
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| Keywords: | Enzymatic hydrolysis Pleurotus ferulae polysaccharide Extraction processes |
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