基于COI条形码序列的珍珠母及其混伪品的DNA分子鉴定

目的：探讨应用COI条形码序列对珍珠母及其混伪品进行物种鉴定的可行性。方法：用MEGA4．0等软件计算珍珠母及其混伪品种内及种问变异，构建珍珠母及其混伪品的NJ树。结果：珍珠母种内COI序列变异小，种问存在较多的变异位点，种问的遗传距离显著大于种内的遗传距离。通过构建的系统聚类树图可以看出，珍珠母不同来源个体均聚在一起，能够与其混伪品区分开。结论：基于COI序列的DNA条形码技术可以很好的鉴定珍珠母的正品来源及其混伪品。

Molecular Identification of Margaritifera concha and its Adulterants Using COI Barcode Sequence

Objective： This study explores the feasibility of using the COI barcode sequence in identifcation of the Margaritifera concha and its adulterants. Methods： Intra- and inter-specific genetic distances of Margaritifera concha and its adulterants have been analyzed by MEGA 4.0 software using COI sequences and the NJ tree has been constructed. Results： Margaritifera concha exhibited low intra-specific sequence variation and the inter-specific sequence have high variation between Margaritifera concha and its adulterants. The inter-specific genetic distances were obviously higher than the intra-specifc ones. The sample of Margaritifera concha clustered together and can separated from its adulterants in the dendrogram constructed. Conclusion： As a DNA barcode, COI sequence can identify the Margaritifera concha with its adulterants.