| 宿主域扩大的苜蓿尺蠖核多角体杂交型病毒 |
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| 引用本文： | 易咏竹,张志芳,肖庆利,何家禄.宿主域扩大的苜蓿尺蠖核多角体杂交型病毒[J].蚕业科学,2001,27(1):75-76. |
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| 作者姓名： | 易咏竹 张志芳 肖庆利 何家禄 |
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| 作者单位： | 农业部家蚕生物技术重点开放实验室，中国农业科学院蚕业研究所，镇江 212018 |
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| 基金项目： | 国家自然科学基金!资助项目 ( 3 9770 5 75 ) |
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| 摘 要： | 将家蚕核型多角体病毒 (BombyxmoriNuclearPolyhedrosisVirus,BmNPV)的解链酶基因DNA和苜蓿尺蠖核型多角体病毒 (AutographacalifonicaMultipleNuclearPolyhedrosisVirus,AcMNPV)的基因组DNA共转染到秋粘虫细胞系Sf 2 1,经过累代的筛选获得既能感染家蚕细胞 ,又能感染秋粘虫细胞的宿主域扩大的杂交型病毒HyNPV。
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| 关 键 词： | 杆状病毒 宿主域 杂交型病毒 |
| 修稿时间： | 2000年10月30 |
The Host range Expanded Hybrid Autographa californica Nuclear Polyhedrosis Virus |
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| Yi,Yongzhu,Zhang,Zhifang,Xiao,Qingli,He,Jialu.The Host range Expanded Hybrid Autographa californica Nuclear Polyhedrosis Virus[J].Acta Sericologica Sinica,2001,27(1):75-76. |
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| Authors: | Yi Yongzhu Zhang Zhifang Xiao Qingli He Jialu |
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| Abstract: | The helicase gene of Bombyx mori Nuclear Polyhedrosis Virus were contransfected into Sf 21 cell with the genomic DNA of Autographa californica Nuclear Polyhedrosis Virus.The hostrange expanded hybrid virus (HyNPV),which could infect both Bm cell line and Sf cell line,was constructed via multiple generation selection. |
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| Keywords: | Baculovirus Host range Hybrid baculovirus |
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