钩藤属部分不同种植物药材对正常大鼠血压的影响

目的:观察钩藤属6个不同种药材乙醇提取物对正常大鼠血压的影响。方法:急性降压试验:将大鼠分为正常对照、硝苯地平0.002 g.kg-1以及钩藤、大叶钩藤、侯钩藤、攀茎钩藤、倒挂金钩、北越钩藤乙醇提取物含生药68.4,34.2 g.kg-114组,大鼠十二指肠给药1次,记录给药前及给药后0.5,1.0,1.5,2.0,2.5,3.0 h大鼠颈总动脉血压;慢性降压试验:将大鼠分为正常对照、硝苯地平0.002 g.kg-1以及前述6种药材乙醇提取物34.2,17.1 g.kg-1组,灌胃,每天1次,连续11 d,给药前和给药后第7天和第11天测定大鼠尾动脉血压。以给药后自身血压下降达2.666 kPa以上,同时与对照组比较有显著性差异者为药物降血压有效。结果:钩藤68.4,34.2 g.kg-1给药后0.5 h颈总动脉血压下降达2.666 kPa以上,且与对照组比较有显著性差异(均P<0.01),并维持3 h以上。钩藤34.2 g.kg-1给药后第7,11天,17.1 g.kg-1给药后第11天;大叶钩藤、侯钩藤34.2 g.kg-1给药后第11天,大鼠尾动脉血压下降值达2.666 kPa以上,且与对照组比较有显著性差异(均P<0.01);而攀茎钩藤、倒挂金钩、北越钩藤给药后大鼠颈总动脉、尾动脉血压下降值均未达到2.666 kPa。结论:钩藤、大叶钩藤、侯钩藤能够显著降低正常大鼠血压,攀茎钩藤、倒挂金钩、北越钩藤对正常大鼠血压没有显著影响。

Effect of Ethanol Extract of Different Plant Breeds Uncaria Schreb on Blood Pressure in Normal Rat

Objective: To investigate the effects of ethanol extract of six breeds of Uncaria on blood pressure in normal rat. Method: For acute hypotensive experiment, the rats were randomly divided into normal control group, nifedipine(0.002 g· kg^-1) group and ethanol extract groups of six breeds of Uncaria Schreb at dose of crude drug 68.4, 34.2 g· kg^-1. The common carotid artery blood pressures were recorded 0, 0.5,1.0,1.5,2.0,2.5,3.0 hours after single dose administration of medicine to duodenum. For chronic hypotensive experiment, the rats were randomly divided into normal control group, nifedipine(0.002 g· kg^-1) group and ethanol extract groups of six breeds of Uncaria Schreb at dose of crude drug 34.2, 17.1 g· kg^-1. The caudal artery blood pressures were recorded on 0, 7,11 days after repeated intragastric administration. Result: Uncaria rhynchophlly(Miq.)Miq.ex Havil at doses of 68.4, 34.2 g· kg^-1 could significantly decrease the common carotid artery blood pressure of normal rats from 0.5 h to 3 h after single dose administration (P<0.01).U. rhynchophlly(Miq.)Miq.ex Havil at doses of 17.1,34.2 g· kg^-1, U. macrophylla Wall and U. rhynchophylloides F.C.How at dose of 34.2 g crude drug· kg^-1could significantly decrease the caudal artery blood pressure of normal rats after repeated intragastric administration (P<0.01). But U. scandens (Sm.)Huntch, U. lancifolia Hutch and U. homomalla Miq could not decrease the common carotid artery and caudal artery blood pressure of normal rat. Conclusion: U. rhynchophlly(Miq.)Miq.ex Havil, U. macrophylla Wall and U. rhynchophylloides F.C.How could significantly decrease the blood pressure of normal rats. But U. scandens (Sm.)Huntch, U. lancifolia Hutch and U. homomalla Miq showed no influence on blood pressure in normal rat.