| 流动注射化学发光测定苹果多酚抗氧化活性 |
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| 引用本文： | 刘彩红,李玉琴,李珂,贾宝秀,郭玉蓉.流动注射化学发光测定苹果多酚抗氧化活性[J].食品工业科技,2012(1):128-130. |
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| 作者姓名： | 刘彩红 李玉琴 李珂 贾宝秀 郭玉蓉 |
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| 作者单位： | 1. 泰山医学院药学院,山东泰安,271000
2. 陕西师范大学食品工程与营养科学学院,陕西西安,710062 |
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| 基金项目： | 农业部现代苹果产业技术体系-苹果采后加工技术研究(Nycytx-08) |
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| 摘 要： | 用流动注射化学发光法测定苹果多酚的体外抗氧化作用。将苹果多酚提取液加入3种化学发光体系,测量其发光强度,根据系统化学发光被抑制的程度评价苹果多酚对活性氧自由基的清除能力,并以抗坏血酸(VC)为阳性对照。结果表明,苹果多酚对三种活性氧自由基(O2-·、·OH、H2O2)的清除能力远强于VC;对H2O2和·OH的清除能力相当,强于对O2-·的清除能力。结果显示苹果多酚具有很好的抗氧化能力。
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| 关 键 词： | 流动注射化学发光 苹果多酚 抗氧化 |
Determination of antioxidation of apple polyphenols by flow injection chemiluminescence |
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| LIU Cai-hong,LI Yu-qin,LI Ke,JIA Bao-xiu,GUO Yu-rong.Determination of antioxidation of apple polyphenols by flow injection chemiluminescence[J].Science and Technology of Food Industry,2012(1):128-130. |
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| Authors: | LIU Cai-hong LI Yu-qin LI Ke JIA Bao-xiu GUO Yu-rong |
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| Affiliation: | 1.Pharmacy Department of Taishan Medical University,Tai’an 271000,China;2.College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an 710062,China) |
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| Abstract: | The purpose was to determine the scavenging ability on ROS free radical of apple polyphenols in vitro by flow injection chemiluminescence.The determination was based on the inhibition effect of apple polyphenols extraction in three chemiluminescence system,and vitamin C was used as positive control.It was showed that apple polyphenols original liquid can inhibit and clear the ROS such as O2-·,·OH and H2O2 effectively,and the scavenging ability was better than vitamin C.The scavenging ability of apple polyphenols on H2O2 was much better than O2-·,but was equal to ·OH.Conclusion was the apple polyphenols extraction was an excellent natural antioxidant. |
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| Keywords: | flow injection chemiluminescence apple polyphenols antioxidation |
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