石菖蒲、安息香和苏合香挥发油促进罗丹明123的肠吸收作用及其机制

目的 研究石菖蒲、安息香和苏合香挥发油促进罗丹明-123 (Rho-123)的肠吸收作用及其机制.方法 制作大鼠肠外翻模型,向肠囊内注入1.0 mL K-R营养液,将肠囊悬浮于45.0 mL 37℃的K-R溶液中.向肠囊外溶液中分别加入浓度均为60 μg/mL的石菖蒲、安息香和苏合香挥发油,孵育30 ain后加入Rho-123,用高效液相色谱-荧光检测法测定肠囊内Rho-123的含量.培养人结肠癌细胞Caco-2,分别加入浓度均为80 μg/mL的石菖蒲、安息香和苏合香挥发油作用48 h,应用流式细胞术检测P糖蛋白(P-gp)的表达;采用qPCR检测P-gp基因MDR1 mRNA的表达.结果 石菖蒲、安息香和苏合香挥发油均可增加大鼠空肠和回肠Rho-123的吸收速率常数和表观通透系数(P＜0.01),降低P-gp及其基因MDR1 mRNA的表达(3者作用的降低率分别为53.15％、55.10％、61.86％及55.41％、16.24％、38.46％;P＜0.01).结论 抑制P-gp及其基因的表达可能是石菖蒲、安息香和苏合香挥发油促进Rho-123肠吸收的主要机制之一.

Promoting effect of volatile oils of Shichangpu, benzoin or storax on intestinal absorption of Rhodamine-123 and its mechanisms

Objective To explore the intestinal absorption-promoting effect of volatile oils of Shichangpu,benzoin and storax on Rhodamine-123 (Rho-123) and its mechanisms.Methods Rat in vitro everted gut sac (EGS) model was made.The EGS was filled with 1.0 mL K-R solution,and suspended in 45.0 mL 37 ℃ K-R solution with or without 60 μg/mL volatile oils of Shichangpu,benzoin or storax.After incubation for 30 min,Rho-123 was added in the experimental solution outside the EGS.Rho-123 content in the EGS was determined by high-performance liquid chromatography-fluorescence detector.The human colon carcinoma cell lines Caco-2 were cultured in DMEM and incubated with 80 μg/mL volatile oils of Shichangpu,benzoin or storax for 48 h.P-glycoprotein (P-gp) expression in Caco-2 cells was assessed using flow cytometry,and P-gp gene MDR1 mRNA expression was determined by qPCR.Results The volatile oils of Shichangpu,benzoin and storax each significantly increased the absorption rate constant and apparent permeability coefficient of Rho-123 in rat jejunum and ileum (P＜0.01),reduced P-gp expression and MDR1 mRNA level in Caco-2 cells (the decreased rates of P-gp expression were 53.15 ％,55.10％ and 61.86％,and of MDR1 mRNA levels were 55.41％,16.24％ and 38.46％,P＜0.01).Conclusion Inhibiting the protein and MDR1 mRNA expressions of P-gp may be one of the main mechanisms of volatile oils of Shichangpu,benzoin and storax in promoting Rho-123 intestinal absorption.