超临界CO2萃取黑莓籽油及其成分分析

目的:以黑莓籽为原料,用超临界CO2萃取法提取黑莓籽油,并测定其成分。方法:采用超临界CO2萃取方法提取黑莓籽油,通过正交试验对影响提取过程的参数进行优化,确定黑莓籽油提取的最佳工艺条件,并用气相色谱-质谱法分析黑莓籽油的脂肪酸组成。结果:超临界CO2流体萃取黑莓籽油的最佳工艺条件为萃取温度35℃、萃取压力30MPa、分离压力12MPa、分离温度55℃,此条件下黑莓籽油的得率达16.10%,其脂肪酸组成为软脂酸5.38%、硬脂酸3.53%、油酸13.70%、亚油酸60.48%、亚麻酸11.16%,总不饱和脂肪酸含量85.3 4%。

Supercritical Carbon Dioxide Extraction and Fatty Acid Composition Analysis of Blackberry Seed Oil

Objective: To extract blackberry seed oil from blackberry seeds with supercritical carbon dioxide and determine its fatty acid composition.Methods: Operating parameters that influence the extraction of blackberry seed oil were optimized using an orthogonal array design.The fatty acid composition of blackberry seed oil was analyzed by gas chromatography-mass spectrometry(GC-MS).Results: The optimal extraction conditions were extraction temperature of 35 ℃,extraction pressure of 12 MPa,separation pressure of 12 MPa and separation temperature of 55 ℃.Under thess conditions,the yield of blackberry seed oil was up to 16.10%.The major fatty acids were palmic acid(5.38%),stearic acid(3.53%),oleic acid(13.70%),linoleic acid(60.48%),gamma-linolenic acid(11.16%) and total unsaturated fatty acid(85.34%).