高效液相色谱法测定保健食品中松果菊苷

目的:建立高效液相色谱法测定保健食品中松果菊苷的方法。方法:样品经50%甲醇提取,以0.2%磷酸-乙腈为流动相,流速为1.0 ml/min,Intersil C18柱分离(柱温为35℃),在331 nm处检测。结果:被测组分在28μg/ml～280μg/ml范围内线性关系良好(r=0.9999);加标回收率为89.3%～101.2%,相对标准偏差(RSD)2.92%～3.50%(n=6)。方法检出限为0.84μg/ml,方法定量限为2.81μg/ml。结论:本方法快速、灵敏、重现性好,适用于保健食品中松果菊苷的测定。

Determination of echinacoside in health foods by HPLC

Objective:An analytical method for determination of echinacoside in health foods by high performance liquid chromatography was established.Methods:The samples were extracted with 50% methanol,then separated on a Intersil C18 column with a mixture of 0.2% phosphoric acid-acetonitrile(85+15,V/V) as mobile phase;the flow rate was 1.0 ml/min.The detection wavelength was 331 nm.Results:Good linearity of the calibration curve between the peak area and the mass of echinacoside was obtained in the range of 28 μg/ml~280 μg/ml(r=0.9999);the relative standard deviations were 2.92%~3.50%(n=6);the recoveries were 89.3%~101.2%.The detection limit and quantification limit of the method were 0.84 μg/ml and 2.81 μg/ml respectively.Conclusion:This method is simple,rapid,precise,accurate and it can be used for determination of echinacoside in health foods.