不同碳氮组合对小球藻异养培养油脂积累的影响

目的探讨不同碳氮组合对小球藻Chlorella pro tothecoides CS-41异养培养油脂积累的影响。方法以不同浓度葡萄糖为碳源,尿素为氮源,异养培养小球藻,采用干重法测定小球藻生物量,氯仿/甲醇法提取总脂,并采用GC-MS分析脂肪酸含量。结果小球藻最佳生长条件的碳氮组合配比为葡萄糖浓度40g/L,尿素浓度3g/L,此时小球藻生物量达最高,为9.1g/L,脂肪酸产率最大,为1.26g/L;总脂含量最高,为4.38g/L。结论获得了碳氮最佳配比,使小球藻脂肪酸含量提高到1.26g/L,为发酵扩大化培养小球藻制备生物柴油奠定了基础。

Effect of Carbon and Nitrogen Sources on Lipid Accumulation of Heterotrophic Microalgae Chlorella protothecoides

Objective To investigate the effect of carbon and nitrogen sources on the lipid accumulation of heterotrophic microalgae Chlorella protothecoides.Methods C.protothecoides was cultured in a heterotrophic mode using various concentrations of glucose as carbon source and urea as nitrogen.The biomass of C.protothecoides was determined by loss on drying.The total lipid was extracted by chloroform/alcohol and analyzed for fatty acid content by GC-MS.Results The optimal concentrations of glucose and urea for growth of C.protothecoides were 40 and 3 g/L respectively,at which the biomass of C.protothecoides,fatty acid yield and total lipid content reached maximums of 9.1,1.26 and 4.38 g/L respectively.Conclusion The formula of carbon and nitrogen sources was optimized,and the fatty acid content of C.protothecoides increased to 1.26 g/L,which laid a foundation of biodiesel preparation by large-scale fermentation of C.protothecoides.