| 固定化黄孢原毛平革菌木素过氧化物酶的研究 |
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| 引用本文： | 吴叶明,张朝晖,刘东华.固定化黄孢原毛平革菌木素过氧化物酶的研究[J].纤维素科学与技术,2008,16(4):12-16,23. |
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| 作者姓名： | 吴叶明 张朝晖 刘东华 |
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| 作者单位： | 浙江工业大学,生物与环境工程学院,浙江,杭州,310032 |
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| 摘 要： | 用大孔吸附树脂进行黄孢原毛平革菌来源的木素过氧化物酶固定化试验,筛选出固定化效果较好的XAD7HP大孔树脂,研究了其固定化条件。结果表明,当树脂1.0g,酶液pH4.5,加酶量87.2U,吸附温度25℃,吸附4h,戊二醛质量分数0.2%,戊二醛处理时间120min,可获得最佳的固定化效果,固定化酶活力可达到16U/g(对载体)。
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| 关 键 词： | 黄孢原毛平革菌 木素过氧化物酶 大孔吸附树脂 固定化 |
Study on Immobilization of Lignin Peroxidase from Phanerochaete chrysosporium Using Resin XAD7HP |
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| WU Ye-ming,ZHANG Zhao-hui,LIU Dong-hua.Study on Immobilization of Lignin Peroxidase from Phanerochaete chrysosporium Using Resin XAD7HP[J].Journal of Cellulose Science and Technology,2008,16(4):12-16,23. |
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| Authors: | WU Ye-ming ZHANG Zhao-hui LIU Dong-hua |
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| Abstract: | Lignin peroxidase (LiP) from Phanerochaete chrysosporium was immobilized on three kinds of microporous absorbent industrial resins. XAD7HP showed the best result for LiP immobilization. The optimal conditions for LiP immobilization were as follow: the weight of the resin,1.0 g; pH 4.5 of the enzyme solution; the amount of LiP used,87.2 U; the temperature and time for the absorption,25℃ and 4 h; glutaraldehyde concentration,0.2 %; the cross-linked time with glutaraldehyde,120 min. Under the optimal conditions,the activity of immobilized enzyme was 16 U/g carrier. |
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| Keywords: | Phanerochaete chrysosporium lignin peroxidase microporous absorbent resins immobilization |
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