Rb和MCM2蛋白在前列腺癌中的表达及临床意义

目的：探讨视网膜母细胞瘤易感基因（Rb）和微染色体维持蛋白2（MCM2）在前列腺癌发病机制中的作用及其临床意义。方法：应用免疫组化EliVision^TMplus二步法，检测49例前列腺癌（PCa）组织，20例良性前列腺增生（BPH）及10例正常前列腺组织（NP）中pRb蛋白与MCM2的表达。结果：pRb在PCa，BPH与NP组织中的阳性表达率分别为44．90％，80％，90％，PCa中pRb的水平明显低于BPH（P〈0．05）及NP（P〈0．05），且与PCa病理分级（P〈0．05）和临床分期（P〈0．05）呈负相关。MCM2在PCa，BPH与NP组织中的阳性表达率分别为67．35％，25％和10％，PCa中MCM2的水平明显高于BPH（P〈0．05）及NP（P〈0．05），且与PCa病理分级（P〈0．05）和临床分期（P〈0．05）呈正相关。MCM2表达与pRb表达呈负相关（rs=-0．596，P〈0．01）。结论：Rb基因参与了前列腺癌的发生、发展过程，检测Rb基因和MCM2有助于判定前列腺癌恶性程度及预后。

Expression and clinical significance of Rb gene and MCM2 in prostate cancer

Objective: To study the expression of retinoblastoma susceptibility gene(Rb) and minichromosome maintenance protein 2(MCM2) in primary prostate cancer(PCa) and its clinical significance.Methods: The expression of MCM2 protein and pRb were detected in 49 cases of PCa,20 cases of benign prostate hyperplasia(BPH),10 cases of normal prostate tissues(NP) by EliVisionTM plus immunohistochemical staining.Results: The positive expression rate of pRb in PCa,BPH,NP were 44.90%,80% and 90% respectively.The expression level of pRb in PCa was significantly lower than that in BPH(P<0.05) and NP(P<0.05),and was negatively correlated with pathological grades(P<0.05) and clinical stages(P<0.05) of PCa.The positive expression rate of MCM2 in PCa,BPH,NP were 67.35%,25% and 10% respectively,however,the expression level of MCM2 in PCa was significantly higher than that in BPH(P<0.05) and NP(P<0.05),and was positively correlated with pathological grades(P<0.05) and clinical stages(P<0.05) of PCa.The expression level of MCM2 in PCa was negatively correlated with pRb(rs =-0.596,P <0.01).Conclusion: Rb might be involved in the occurrence and development of PCa.The determination of Rb and MCM2 can be an index for estimating the level of malignancy and prognosis of PCa.