| 改进的细菌转化试验方法 |
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| 引用本文： | 曹明富.改进的细菌转化试验方法[J].杭州师范学院学报(社会科学版),1990(6). |
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| 作者姓名： | 曹明富 |
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| 作者单位： | 杭州师范学院生物系 |
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| 摘 要： | 本文对“细菌转化实验方法”进行了改进。用改良碱酶法制备的质粒DNA(PBR322)作供体,转化经氯化钙处理的受体菌E.Coli C600,使后者获得抗氨苄青霉素和抗四环素的特性。改进后的转化方法操作简便,不受设备限制。
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| 关 键 词： | 转化 实验方法 质粒DNA 转化子 大肠杆菌 |
A MODIFIED METHOD OF TRANSFORMING BACTERIUM WITH PLASMID DNA |
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| Cao Mingfu.A MODIFIED METHOD OF TRANSFORMING BACTERIUM WITH PLASMID DNA[J].Journal of Hangzhou Teachers College(Humanities and Social Sciences),1990(6). |
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| Authors: | Cao Mingfu |
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| Affiliation: | Department of Biology |
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| Abstract: | We have made further imoprovement in "the experimental method of transforming bacterium." With the method combined by alkali and enzyme to prepare E.coli plasmid DNA (pBR322) donor was used. The pBR322 plasmid DNA was transformed into recipient E.coli C600 competents cells with incubation in calcium chloride. The transformants revealed the characteristic of Amp and Tc resistance. The improved method is easy to operate and not restricted by equipment. |
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| Keywords: | transformation experimental method plasmid DNA transfor- mant E coli |
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