| 泥鳅和大鳞副泥鳅性腺细胞H－Y抗原的检测 |
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| 引用本文： | 常重杰,杜启艳,路淑霞,卢龙斗. 泥鳅和大鳞副泥鳅性腺细胞H－Y抗原的检测[J]. 河南师范大学学报(自然科学版), 1994, 0(3) |
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| 作者姓名： | 常重杰 杜启艳 路淑霞 卢龙斗 |
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| 作者单位： | 河南师范大学生物系 |
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| 摘 要： | 以雄性小鼠脾细胞为抗原，免疫Ｂａｌｂ／ｃ小鼠，获得了高滴度的抗Ｈ－Ｙ抗原抗体．在此基础上，利用细胞毒性实验，对泥鳅和大鳞副泥鳅的性腺细胞进行了Ｈ－Ｙ抗原的检测．结果表明，泥鳅中雌雄性腺细胞均含有Ｈ－Ｙ抗原；大鳞副泥鳅中，Ｈ－Ｙ抗原仅存在于雌性性腺细胞中，提示其为ＺＺ／ＺＷ型性别决定．
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| 关 键 词： | H－Y抗原，细胞毒性实验，泥鳅，大鳞副泥鳅 |
Detection on the H-Y Antigen in Sex Gland Cell of Misgurnus Anguillicaudatusand Paramisgurnus Dabryanus |
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| Chang Zhongjie Du Qiyan Lu Shuxia Lu Longdou. Detection on the H-Y Antigen in Sex Gland Cell of Misgurnus Anguillicaudatusand Paramisgurnus Dabryanus[J]. Journal of Henan Normal University(Natural Science), 1994, 0(3) |
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| Authors: | Chang Zhongjie Du Qiyan Lu Shuxia Lu Longdou |
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| Abstract: | The spleen cell of the male Balb/C mouse immunized the female mice.The high titreantiserum to H-Y antigen were prepared with the H-Y antiserum. We detected the H-Y antigen dis-tribution in sex gland cell of Misgurnus anguillicaudatus and Paramisgurnus dabryanus.The results show-ed that the male and the female have the H-Y antigen in Misgurnus anguillicaudatus, but in Paramisgurnus dabryanus,only the female has the H-Y antigen. |
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| Keywords: | H-Y antigen Misgurnus anguillicaudatus Paramisgurus dabryanus |
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