适配体电化学传感器检测汞离子进展

适配体是一小段经体外筛选得到的寡核苷酸序列。适配体中的胸腺嘧啶(T)碱基可与Hg~(2+)形成比双链DNA更加稳定的T-Hg~(2+)-T结构。利用该性质结合电化学测量方法可制作检测Hg~(2+)的特异性强、灵敏度高的适配体电化学传感器,并建立微量Hg~(2+)的检测方法。该文对近年来发展的检测Hg~(2+)的适配体电化学传感器进行了综述和总结,对文献报道的几类传感器的构建过程和检测机理进行了详述,对检测方法的优缺点进行了分析。最后,对此类传感器今后的发展方向提出了展望,引用文献83篇。

Progress on Detection of Mercury Using Aptamer Electrochemical Sensor

Aptamer is a subparagraph ligonucleotide sequence screened in vitro,and has a high affinity and strong specificity to combine with corresponding ligands.Thymine(T) bases can combine specially with Hg2+ to form the structure of T-Hg2+-T,which is more stable than the double helix of DNA.Based on this structure,an aptamer electrochemical sensor was fabricated to detect Hg2+,and it showed the advantages of high specificity,stability and sensitivity.In this paper,the developed aptamer electrochemical sensors for Hg2+ detection in recent years are summarized and reviewed.The fabrication processes and the detection mechanisms of this kind of sensors are reported in detail,the merit and demerit of the aptamer electrochemical sensor are analyzed.Finally,the developoing prospect for the sensors in the future are put forward,and 83 references are cited.