大鼠急性坏死性胰腺炎外周血中的TNFa,IL-6浓度的动态测定及意义

目的：探讨急性胰腺时外周血清TNFa，IL—6的动态变化及其意义。方法：用chetty法制作大鼠的ANP模型，术后动态测定对照组、ANP组及大黄素治疗组血淀粉酶，并取胰组织作病理检查，同时动态检测外周血清中TNFa，IL—6的情况。结果：血清淀粉酶在0h三组间无差别；24h治疗组、ANP组与对照组间有显著差异(P＜0．01)；36h治疗组与ANP组之间无显著差异(P＞0．05)；二组与对照之间有显著差异(P＜0．01)；24h治疗组、ANP组与对照组间有显著异(P＜0.01)；TNFa，IL-6在0h三组间无差别；24h时治疗组与ANP无统计学差别与对照组比较有明显的升高(P＜0．05)；30h及36h治疗组与ANP组之间有显著差异(P＜0．01)。结论：细胞因子参与急性胰腺炎的发病过程，又与其病情严重程度呈正相关，表明细胞因子在ANP的发生和发展过程中起着一定作用。

DYNAMIC MEASUREMENT AND SIGNIFICANCE OF TNFa,IL-6 IN PERIPHERAL VEINOUS BLOOD IN RATS WITH ACUTE NECROTIZING PANCREATITIS

Objective:To investigate dynamic measurement and significance of TNFa,IL-6 in peripheral veinous blood in rats with acute necrotizing pancreatitis(ANP).Methods:We divided 48 Wister rats randomly into shamoperated group(N=12),ANP model group (n=18) and Emodin-treated group (N=18).ANP models were made by applying Chetty method.These rats were killed respectively at 0h,24h,30h,and 36h after surgery .The contents of serum amylase and the pancreatic histologic changes were observed ;TNFa and IL-6 in peripheral veinous blood were measured.Results:At 0h,the levels of serum amylase and TNFa and IL-6 was not significantly different between groups.At 24h,the levels of serum amylase and TNFa and IL-6 in emodin-treated rats increased greatly compared with those of sham-opreated group and had no significant difference compared with ANP rats respectively. At 30h and 36h,pancreatic pathological degrees in Emodin-treated group were significantly different from those in ANP model group.Conclusion:The concentration of TNFa and IL-6 in peripheral veinous blood is related to the severity of acute necrotizing pancreatitis,which suggests that TNFa and IL-6 contribute to the pathologic process of acute necrotizing pancreatitis.