以水稻秸秆为基质里氏木霉产酶条件优化

以里氏木霉（Trichoderma reesei）为研究对象，对水稻秸秆进行糖化试验。通过单因素试验及响应面法优化里氏木霉产酶培养基及产酶条件。结果表明，里氏木霉产酶最佳培养基为：水稻秸秆15.0 g/L、（NH4）2SO4 2.0 g/L、KH2PO4 3.0 g/L、MgSO4·7H2O 0.5 g/L、吐温-80 0.5 mL/L、微量元素液10.0 mL/L、FeSO4·7H2O 0.005 g/L。此优化条件下，菌株的滤纸酶酶活为0.612 PFU/mL，提高了52.6%。最佳发酵条件为：发酵温度29 ℃，初始pH 6、接种量5.0%、转速150 r/min、发酵时间8 d。在此优化条件下，滤纸酶酶活为1.12 PFU/mL，提高了83.2%。

Optimization of enzyme-producing conditions of Trichoderma reesei with rice straw as substrate

Using Trichoderma reesei as research object, the saccharification test of rice straw was carried out. The enzyme production medium and conditions of T. reesei were optimized by single factor tests and response surface methodology. The results showed that the optimum medium for enzyme production by T. reesei was as follows: rice straw 15.0 g/L, (NH4)2SO4 2.0 g/L, KH2PO4 3.0 g/L, MgSO4·7H2O 0.5 g/L, Tween-80 0.5 ml/L, trace element liquid 10.0 ml/L, and FeSO4·7H2O 0.005 g/L. Under the optimum conditions, the filter paper enzyme activity of the strain was 0.612 PFU/ml, which increased by 52.6%. The optimum fermentation conditions were obtained as follows: fermentation temperature 29 ℃, initial pH 6, inoculum 5.0%, speed 150 r/min, and fermentation time 8 d. Under the optimum conditions, the filter paper enzyme activity was 1.12 PFU/ml, which increased by 83.2%.