光化学法诱导大鼠单侧海马梗死模型的实验研究

目的：观察不同浓度虎红联合冷光源诱导的大鼠单侧海马梗死模型，了解梗死后学习记忆功能及组织病理改变。方法：大鼠60只随机分为8组：单纯光照组，单纯光敏剂组及6组不同光敏剂浓度(30、40、50、60、70、80mg/kg)的模型组，不同光敏剂浓度的模型组给予静脉注射虎红，冷光源照射左侧海马CA1区，3d后观察其梗死面积、神经行为学及组织病理学改变，以评估模型的可行性。结果：50mg/kg以上的虎红经冷光源照射，可诱导大鼠海马梗死，且面积恒定，病理形态学改变明显，但大鼠单侧海马梗死后的神经行为学改变不明显。结论：虎红结合冷光源诱导的大鼠单侧海马梗死模型稳定性、重复性好，动物存活率高。

A model of photochemistry inducing unilateral hippocampal infarction in rats

Objective:To assess the unilateral hippocampal infarction models induced by cold light irradiation companioned by various dose of Rose Bengal based on the changes in neurobehavior and histopathology in the rats. Method:60 rats were divided into eight groups at random: a cold light irradiation group, a photosensitizer group and 6 groups of light irradiation plus photosensitizer(with concentration of 30,40,50,60,70and 80mg/kg respectively).The 6 groups were given cold light irradiationon to CA1 region of left hippocampus immediately after the intravenous injection of Rose Bengal.The availability of model was evaluated by the changes in neurobehavior, pathomorphology and the sizes of infarction 72 hours later.Result: The cerebral infractions were induced by over 50 mg/kg Rose Bengal and the infarction sizes were stable. The changes were pronounced in pathomorphology but unsharp in neurobehavior.Conclusion:The unilateral hippocampal infarction model induced by Rose Bengal associated with cold light irradiation has a good stability, excellent repetition and high survival rate in rats.