丹皮酚磺酸钠对钙反常培养乳鼠心肌细胞Ca2+内流的抑制作用

培养乳鼠心肌细胞复制钙反常模型,观察丹皮酚磺酸钠对心肌细胞⁴⁵Ca摄取、膜唾液酸(SA)含量及细胞搏动频率的影响,结果提示丹皮酚磺酸钠在50～400μg/ml范围内能显著抑制正常心肌细胞快相(5 min)和慢相(120 min)⁴⁵Ca摄取及其搏动频率,且能显著抑制钙反常心肌细胞的⁴⁵²⁺内流外,尚与其提高膜SA含量有关。

THE INHIBITORY EFFECTS OF SODIUM PAEONOL SULFATE ON CALCIUM INFLUX IN THE CULTURED NEONATAL RAT HEART CELLS DURING CALCIUM PARADOX

The calcium paradox model was made in cultured neonatal rat heart cells. The 45Ca uptake by the cultured neonatal rat heart cells and the contents of sialic acid (SA) of the cultured heart cells were measured to observe and evaluate the protective effects of sodium paeonol sulfate against calcium paradox in the cultured heart cells. The results demonstrated that sodium paeonol sulfate 50-400 micrograms/ml remarkably inhibited the rapid (5 min) and the slow (120 min) phases of 45Ca uptake by the cultured heart cells and myocardial beating rate (MBR) under normal states. Besides inhibition of 45Ca uptake by the cultured heart cells, this drug was found to elevate the contents of SA in the membrane of the cultured heart cells during calcium paradox. This indicates that the mechanism of protection against calcium paradox by sodium paeonol sulfate may be associated with blocking the slow calcium channel as well as increasing SA in the cell membrane.