麻醉方法对大鼠生理、血气及血液流变学的影响 |
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引用本文: | 尤国兴,王瑛,王波,尹玉静,赵莲,周虹.麻醉方法对大鼠生理、血气及血液流变学的影响[J].中国输血杂志,2013,26(5):417-420. |
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作者姓名: | 尤国兴 王瑛 王波 尹玉静 赵莲 周虹 |
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作者单位: | 军事医学科学院野战输血研究所,北京,100850 |
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基金项目: | 国家自然科学基金面上基金项目,国家高技术研究发展计划主题项目,北京市科委首都市民健康项目培育 |
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摘 要: | 目的比较不同麻醉方式对大鼠生理、血气及血液流变学的影响,完善血液代用品标准评价模型的制备。方法 Wistar大鼠(16只)随机分为2组,分别采用戊巴比妥钠腹腔注射和乌拉坦肌肉注射的方法麻醉。记录麻醉起效时间,每隔5 min记录各项生理指标,直至术后40 min,随后抽血检测血气、血细胞及各项流变学参数。结果戊巴比妥钠组和乌拉坦组麻醉起效时间分别约为3.67和31 min,戊巴比妥钠腹腔注射起效显著快于乌拉坦肌肉注射(P<0.01);相对于戊巴比妥钠组,乌拉坦组降低平均动脉压(5、15、30、35、40 min)、收缩压(35、40min)、舒张压(15、30、35、40 min)、脉压差(40 min)和体温(除5 min时间点外),均为P<0.05;术后40 min,乌拉坦组Hct高于戊巴比妥钠组(P<0.05),pH明显降低(P<0.01),血浆粘度降低(P<0.05)。结论乌拉坦肌肉注射麻醉影响大鼠生理、血气分析和血液流变学指标,在进行药效评价时需慎重选择。
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关 键 词: | 麻醉 动物模型 平均动脉压 血液流变学 |
The influence of different anesthesia on the physiology, blood gas and hemorheology of rat |
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Abstract: | Objective To compare the influence of different anesthesia on parameters of physiology,blood gas and hemorheology,and standardize the model used for evaluating the blood substitutes.Methods A total of 16 Wistar rats were divided into 2 groups randomly according to the anesthesia,sodium pentobarbital intraperitoneal injection(S group) or urethane intramuscular injection(U group).The acting time was recorded and the physiological parameters were also evaluated every 5 min till 40 min after operation.Then blood was withdrawn to assess the blood gas,blood cells and hemorrheology parameters.Results The times needed for S group and U group were 3.67 min and 31 min respectively,showing that the former came into effect much faster than the latter(P < 0.01).Compared with S group,the U group decreased the mean arterial pressure(5,15,30,35 and 40 min,P < 0.05),the systolic pressure(35 and 40 min,P < 0.05),the diastolic blood pressure(15,30 and 35 min,P < 0.05),the pulse pressure(40 min,P < 0.05) and the body temperature(expect for 5 min,P < 0.05) significantly.And 40 min after the operation,there were significant higher haematocrit(P < 0.05),lower arterial pH value(P < 0.01) and plasma viscosity(P < 0.05) in U group than those in S group.Conclusion The anesthesia via urethane intramuscular injection should be chosen cautiously because of its influences on rats' physiology,blood gas and hemorrheology parameters. |
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Keywords: | anesthesia animal model mean arterial pressure hemorheology |
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