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橄榄叶黄酮类抗氧化物质的结构分析研究
引用本文:刘玉红,林庆生,孔慧清,张晨,李雄,卢秋雁,刘美玲.橄榄叶黄酮类抗氧化物质的结构分析研究[J].食品与发酵工业,2006,32(9):28-31.
作者姓名:刘玉红  林庆生  孔慧清  张晨  李雄  卢秋雁  刘美玲
作者单位:1. 广东省微生物研究所,广州,510070;广东天辰生物技术有限公司,广州,510070
2. 华南理工大学轻工与食品学院,广州,510640
3. 广东天辰生物技术有限公司,广州,510070
摘    要:通过超声强化提取法提取橄榄叶黄酮,采用聚酰胺柱层析和制备薄层层析对橄榄叶黄酮提取液进行分离纯化,通过抗氧化活性比较,筛选抗氧化活性最好的样品C3,运用高效液相色谱法(HPLC)、紫外光谱法(UV)、红外光谱法(IR)、液质联用法(LC-MS)、核磁共振法(1HNMR,13CNMR),对样品C3进行结构分析,确定为木犀草素。

关 键 词:橄榄叶  黄酮  抗氧化  分离纯化  结构分析
收稿时间:06 6 2006 12:00AM
修稿时间:08 9 2006 12:00AM

Study on the Analysing Structure of Flavonoids Antioxidants from Olive Leave
Liu Yuhong,Lin Qingsheng,Kong Huiqing,Zhang Chen,Li Xiong,Lu Qiuyan,Liu Meiling.Study on the Analysing Structure of Flavonoids Antioxidants from Olive Leave[J].Food and Fermentation Industries,2006,32(9):28-31.
Authors:Liu Yuhong  Lin Qingsheng  Kong Huiqing  Zhang Chen  Li Xiong  Lu Qiuyan  Liu Meiling
Affiliation:1 Guangdong Institute of Microbiology, Guangzhou 510070, China; 2 Guangdong TianChen Biotechnology Co., Ltd., Guangzhou 510070, China; 3 College of Light Industry and Food Sciences, South China University of Technology3, Guangzhou 510640
Abstract:The flavonoids of olive leaves was extractied by ultrasonic enhanced extraction,then separated and purified with polyamide column chromatography and PTLC.Sample C3 was selected by screen the antioxidative activity and its structure was elucidated by UV,IR,LC-MS,()~1HNMR,()~(13)CNMR,and was identified as Luteolin.
Keywords:olive leave  flavonoids  anti-oxidation separated and purified  structure elucidated
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