微卫星(TATG)n基序在香菇菌种中的验证

以（TATG）4重复序列为引物对香菇属的3个种13个菌株的微卫星区DNA进行PCR扩增，15%的琼脂糖凝胶电泳，获得了25个条带，并且在供试菌株上表现出多态性，可以实现遗传分类研究。为了验证微卫星分子标记实验准确性，又用RAPD技术对13个供试菌株进行了实验。7个引物在13个菌株上共获得了102条多态性条带。通过聚类分析，RAPD获得的分类结果与微卫星分子标记获得的结果一致。此外，为了证明微卫星分子标记获得的条带不是假阳性，在实验中回收了No.10菌株的PCR扩增产物，进行克隆测序。测序结果显示有（TATG）n基序存在，并且达到了微卫星基序重复数量的最低限度。通过本实验可知，香菇中是存在微卫星（TATG）n基序的, 且基序的多态性可以用于香菇的遗传分类研究。

Microsatellite Motif (TATG)n in Lentinula edode

The first microsatellite motif (TATG) n in cultivated edible fungi from Agaricus bisporus was tested in Lentinula edode strains. A 16 mer oligonucleotide (TATG) 4 was synthesized and used as primer in PCR with the DNA of 13 Lentinula edode strains as template.25 DNA fragments were amplified, and the strains were identified according to their polymorphs. Bands of No.10 strain were obtained from 1 5% agarose gel, sequenced, indicating that (TATG) 4 repeats are present in Lentinula edode . The strains were also tested by RAPD technique and 102 DNA fragments were amplified by 7 random primers. The cluster analysis results proved the results by Microsatellite. The variability found within closely related strains indicates that such microsatellites are useful in studying genetic variability in wild and cultivated Lentinula edode .