2,4-二氯苯酚低浓度长期暴露对鲫鱼肝脏抗氧化系统的影响

在室内模拟条件下,研究了低浓度2,4-二氯苯酚(2,4-DCP)长期暴露(40d)对鲫鱼(Carassius auratus)幼体肝脏抗氧化系统的影响结果表明:过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶(Se-GPx)活性、氧化型谷胱甘肽(GSSG)含量可被显著诱导;超氧化物歧化酶(SOD)活性在0.005mg·L^-1 2,4-DCP污染胁迫下即被显著诱导;还原型谷胱甘肽(GSH)含量几乎持续受到抑制;谷胱甘肽还原酶(GR)活性则先受到抑制后逐渐回升;谷胱甘肽硫转移酶(GST)的活性变化较小,仅在2个低浓度组中有轻微诱导.GR、GSH,尤其是SOD,对2,4-DCP较为敏感,可以作为水生生态系统中2,4-DCP污染的一项早期监测指标.

Effects of Long-term Exposure of Low-level 2,4-Dichlorophenol on the Antioxidant Defense System in Liver of Carassius auratus

Under the indoors simulant conditions, the effects of long-term exposure of low-level 2,4-Dichlorophenol on the antioxidant system of larval fishes were studied. The results showed that the activities of catalase (CAT), sedium-dependent glutathione peroxidase (Se-GPx) and the content of oxidized glutathione (GSSG) were induced significantly. The activity of superoxide dismutase (SOD) was induced remarkably when 2,4-Dichlorophenol level was at only 0.005 mg.L-1; the content of reduce glutathione (GSH) was almost suppressed continuously; the activity of glutathione reductase was inhibited firstly and then restored gradually; the activity of glutathione S-transferase (GST) had slight variances among groups. GR, GSH, especially SOD, can act as an early monitoring index of 2,4-Dichlorophenol pollution in aquatic ecosystem.