放大培养对岩黄连细胞悬浮培养的影响

为了优化岩黄连细胞悬浮培养的条件,研究了在放大培养过程中,岩黄连细胞生长与主要营养成分的代谢动力学,以及生物碱的产生情况。结果表明,在不同培养体系下,细胞生长曲线均呈现"S"型。随着培养体积从50、100 mL放大到500 mL和1 L,培养液中碳源、氮源和磷源的消耗减慢,细胞生物量减少,生物碱产量降低。其中100 mL培养体系所获生物量最高,达到15.2 g/L,生物碱产量也最高,脱氢卡维丁为8.35 mg/mL,小檗碱为4.58 mg/mL。根据本文结果,提出了岩黄连细胞培养条件的优化和大规模细胞培养生产岩黄连生物碱应采取的策略。

Effects of Scale-up Cultivation on Cell Suspension Cultures of Corydalis saxicola Bunting

In scale-up cell suspension cultures of Corydalis saxicola Bunting,cell growth,nutrient consumption and alkaloid productions were investigated to optimize the culture conditions.The results showed that although cells were cultivated at different conditions,the growth curves were all "S" shaped.Total carbohydrate,nitrate and phosphate were assimilated gradually throughout the growth period,and the nutrient consumptions were depressed with the culture volume increased from 50 and 100 mL to 500 mL and 1 L,respectively.The scale-up cultures also resulted in decrease of biomass and alkaloid productions.The highest biomass and productions of dehydrocavidine and berberine were obtained in 100-mL cultures,with 15.2 g/L,8.35 mg/mL and 4.58 mg/mL,respectively.According to the results,strategies were forwarded to optimize culture condition and to promote alkaloid productions by large-scale of C.saxicola cells.