| 浅蓝菌素在诱导骨肉瘤细胞株U2-OS凋亡过程中对Bcl-2和Bax的影响 |
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| 引用本文： | 黄伟,舒勇,黄山虎,刘志礼,解志波,刘先明.浅蓝菌素在诱导骨肉瘤细胞株U2-OS凋亡过程中对Bcl-2和Bax的影响[J].江西医学院学报,2009,49(3):35-37. |
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| 作者姓名： | 黄伟 舒勇 黄山虎 刘志礼 解志波 刘先明 |
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| 作者单位： | [1]南昌大学研究生院医学部2006级,南昌330006 [2]南昌大学第一附属医院矫外科,南昌330006 [3]萍乡市人民医院矫外科,江西萍乡337002 |
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| 摘 要： | 目的研究凋亡蛋白Bcl-2、Bax是否参与浅蓝菌素（Cerulenin）诱发骨肉瘤细胞株U2-OS凋亡信号传导。方法采用Western—blotting检测Cerulenin作用前、后骨肉瘤细胞株U2—OS中凋亡蛋白Bcl-2和Bax表达变化情况，了解不同的Cerulenin剂量和作用时间骨肉瘤细胞株U2-OS中凋亡蛋白Bcl-2和Bax表达变化情况。结果骨肉瘤细胞株U2-OS中Bcl-2和Bax呈阳性表达，Cerulenin作用细胞株后Bcl-2表达量随着浓度增加和作用时间延长而递减，Bax表达量随着浓度增加和作用时间延长而递增。结论Cerulenin可能通过下调Bcl-2的表达和上调Bax的表达而诱导骨肉瘤细胞凋亡。
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| 关 键 词： | 浅蓝菌素 Bcl-2 Bax 凋亡 |
Effect of Cerulenin-induced Apoptosis on Bcl-2 and Bax in U2-OS Cells |
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| HUANG Wei,SHU Yong,HUANG Shan-hu,LIU Zhi-li,XIE Zhi-bo,LIU Xian-ming.Effect of Cerulenin-induced Apoptosis on Bcl-2 and Bax in U2-OS Cells[J].Acta Academiae Medicinae Jiangxi,2009,49(3):35-37. |
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| Authors: | HUANG Wei SHU Yong HUANG Shan-hu LIU Zhi-li XIE Zhi-bo LIU Xian-ming |
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| Affiliation: | HUANG Wei ,SHU Yong ,HUANG Shan-hu ,LIU Zhi-li ,XIE Zhi-bo, LIU Xian-min (1. 2006 Grade of Medical Department of Graduate School; 2. Department of Orthopaedics ,the First Affiliated Hospital, Nanchang University, Nanchang 330006, China ; 3. Department of Orthopaedics, Pingxiang People ＇ s Hospital, Pingxiang 337002, China) |
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| Abstract: | Objective To investigate the apoptosis in osteosarcoma cell line U2-OS induced by Cerulenin,and its relationship with Bcl-2 and Bax. Methods Western blotting was used to determine the expression of apoptosis-related protein Bcl-2 and Bax, pre-and post administration of Cerulenin with different doses and length of time. Results There were positive expressions of Bcl-2 and Bax in U2-OS. The expression of Bcl-2 was in inverse proportion to the concentration of Cerulenin and its acting time, while the expression of Bax was in direct proportion to them. Conclusion The apotosis of osteosarcoma cell line U2-OS induced by Cerulenin may be mediated by down-regulation of the expression of Bcl-2 and up-regulation of the expression of Bax. |
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| Keywords: | Bcl-2 Bax |
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