喉鳞状细胞癌中MMP-1和TIMP-1表达的临床病理学意义

目的探讨喉鳞状细胞癌（LSCC）组织中基质金属蛋白酶-1（MMP-1）和基质金属蛋白酶抑制因子-1（TIMP-1）蛋白和mRNA的表达及其临床病理学意义。方法分别应用免疫组织化学和RT-PCR方法 ,检测喉鳞状细胞癌组织中MMP-1和TIMP-1的蛋白及mRNA表达情况。结果 MMP-1在喉鳞状细胞癌组织和癌旁组织中的蛋白表达阳性率比较,差异有统计学意义（P﹤0.05）,且MMP-1 mRNA在喉鳞状细胞癌组织中的表达高于癌旁组织（P﹤0.05）;TIMP-1在喉鳞状细胞癌组织和癌旁组织中的蛋白表达阳性率,差异有统计学意义（P﹤0.05）且TIMP-1 mRNA在喉鳞状细胞癌组织中的表达低于癌旁组织（P﹤0.05）。结论 MMP-1和TIMP-1表达与喉鳞状细胞癌的发生发展﹑浸润及转移有关,检测癌组织中的MMP-1和TIMP-1的基因及蛋白表达情况,有助于判断喉鳞状细胞癌的转移、TNM分期及预后。

The clinical pathological significance of the expression of matrix metalloproteinase 1 and tissue inhibitor of metalloproteinase 1 in laryngeal squamous cell carcinoma

Objective To investigate the protein and mRNA expression of matrix metalloproteinase-1 （ MMP-1） and tissue inhibitor of metalloproteinase-1 （ TIMP-1） in laryngeal squamous cell carcinoma （ LSCC） . Methods Immunohistochemistry SP method and RT - PCR were used respectively to detect the protein and mRNA expression of MMP-1 and TIMP-1 in laryngeal squamous cell carcinoma. Results The positive protein expression of MMP-1 in tumor tissues of LSCC （ 82. 4% ） was significantly higher than that in corresponding non-cancerous tissues （ 18. 2% ） （ P ﹤ 0. 05） ,and mRNA expression of MMP-1 in tumor tissues was significantl y higher than that in corresponding non-cancerous tissues （ P ﹤ 0. 05） . The positive protein expression of TIMP-1 in the tumor tissues of LSCC （ 72. 5% ） was obviously lower than that in corresponding non-cancerous tissues （ 95. 5%） （ P ﹤ 0. 05） ,and mRNA expression of TIMP-1 in tumor tissues was remarkably lower than that in corresponding non-cancerous tissues （ P ﹤ 0. 05） . The protein and mRNA expression of MMP-1 and TIMP-1 was correlated with the development of laryngeal squamous cell carcinoma,grade and regional nodal metastasis （ P ﹤ 0. 05） . Conclusion MMP-1 and TIMP-1 expression is associated with the development,infiltration and metastasis of laryngeal squamous cell carcinoma.