产葡萄糖氧化酶黑曲霉紫外诱变及产酶条件的研究

目的提高黑曲霉中葡萄糖氧化酶的产量。方法紫外诱变法选出产酶优势菌株，优化碳源、氮源、碳酸钙、发酵时间等条件。结果黑曲霉发酵液酶活达到6．5U／mL，比初始酶活提高5倍；单因素条件试验表明，最适碳源是蔗糖，最适氮源是蛋白胨和NaNO3，发酵周期为48h，培养温度是30℃，液体培养基的初始pH值为6．0产酶效果最好。结论紫外诱变后，葡萄糖氧化酶的活力有明显提高。

Research on UV Mutation of Aspergiilus niger and Glucose Oxidase-producing Conditions

Objective To improve the production of glucose oxidase from Aspergillus niger. Methods The dominant bacterial strain was selected through UV mutation, and the conditions were optimized, including carbon source, nitrogen source, calcium carbonate, fermentation time and so on. Results The enzyme activity of Aspergillus niger fermentation liquid was 6.5 U/ml, which was five times higher than initial bacterial strain. The single factor test dedicated that the optimal carbon source was sucrose, nitrogen source was peptone and sodium nitrate, the pH of liquid medium was about 6.0, and the fermentation cycle was 48h at 30℃. Conclusion The vitality of glucose oxidase can be obviously improved through UV mutation of Aspergillus niger.