项丛刺针法对缺血性脑卒中大鼠脑微循环障碍的影响

目的:观察项丛刺针法对缺血性脑卒中后遗症期大鼠脑软膜微循环血流量和全血黏度流变学的影响,探讨针刺作用的可能机制。方法:Wistar大鼠随机分为假手术组、模型组、西药组、针刺组,每组20只。采用线栓法阻断大脑中动脉制备大鼠脑缺血模型。针刺组大鼠在成功造模5周后采用项丛刺针法进行治疗,每日1次,共治疗2周;西药组大鼠用尼莫地平灌胃,每日1次,疗程同针刺组。应用激光多普勒血流仪测定大鼠脑微循环血流量,自动血流变测试仪测定大鼠全血黏度。结果:与假手术组相比,模型组大鼠脑软膜微循环血流量显著降低(P<0.01),全血黏度显著增高(P<0.01)。与模型组相比,针刺组与西药组均可显著增加大鼠脑软膜微循环血流量和降低血液黏度(P<0.01)。与西药组相比,针刺组效果更显著(P<0.01)。结论:项丛刺针法能显著改善缺血性脑卒中后遗症大鼠脑微循环障碍。

Effect of Nape Cluster-acupuncture Treatment on Brain Microcirculation in Ischemic Stroke Rats

Objective To observe the effect of nape cluster-acupuncture treatment on blood microcirculation of the ce-rebral piamater and the whole blood viscosity in cerebral ischemia(CI) rats so as to study its mechanism underlying improvement of CI.Methods Eighty male Wistar rats were randomly allocated to false operation group,CI model group,medication group and acupuncture group(n=20).CI model was established by occlusion of the middle cerebral artery with intraluminal suture-ball blocking method.For rats of the nape cluster-acupuncture group,acupuncture needles were inserted into "Fengfu"(GV 16),"Yamen"(GV 15),"Xia-naohu"(GV 17),"Fengchi"(GB 20),and the points of evenly divided into three parts between GV 16 and GB 20 respectively,and rotated for 1 min,then retained for 15 min,once a day,for 14 sessions.Rats of the medication group were given with intragatric perfusion of nimodipine(12 mL/kg),once daily for 14 days.The blood microcirculation of the brain was detected by using a laser Doppler flowmeter with the probe put near the surface of cerebral piamater.The whole blood viscosity was determined using a blood rheological automatic detector.Results In comparison with the false operation group,the blood flow volume of the cerebral piamater was remarkably decreased and the whole blood viscosity level significantly increased in the model group(P<0.01).In comparison with the model group,the blood flow volume of the cerebral piamater was obviously increased and the whole blood viscosity apparently decreased in both the acupuncture and medication groups(P<0.01).The effects of acupuncture were obviously better than those of the medication in upregulating blood flow volume and in lowering whole blood viscosity(P<0.01).Conclusion The nape cluster acupuncture treatment has a favorable role in improving brain microcirculation by raising blood flow volume and reducing blood viscosity in ischemic stroke rats.