lncRNA ABHD11-AS1通过调控STAT1/STAT3表达促进非小细胞肺癌细胞增殖与迁移

  目的   探讨lncRNA ABHD11-AS1在非小细胞肺癌中的生物学功能和作用机制。  方法   选取2016年7月至2018年12月在广东医科大学附属医院行非小细胞肺癌手术的248例患者，采用χ2检验分析lncRNA ABHD11-AS1的表达与患者年龄、性别、临床病理分期、病理类型及吸烟状态的关系；采用Kaplan-Meier法分析ABHD11-AS1对非小细胞肺癌患者的预后意义；应用qRTPCR方法检测ABHD11-AS1在非小细胞肺癌组织和细胞系中的表达；通过体外功能测定评估敲低ABHD11-AS1对细胞增殖和转移的影响；通过Western blot实验探讨ABHD11-AS1在非小细胞肺癌中致癌作用的分子机制。  结果   lncRNA ABHD11-AS1高表达组较低表达组吸烟患者较少（P=0.02），分期更晚（P < 0.01）；ABHD11-AS1高表达预示非小细胞肺癌患者预后不良；ABHD11-AS1在非小细胞肺癌组织和癌细胞株中高表达；敲低ABHD11-AS1的表达可抑制体外细胞增殖和迁移；敲低ABHD11-AS1表达抑制STAT1和STAT3癌蛋白的表达。  结论   ABHD11-AS1可能通过促进STAT1和STAT3癌蛋白的表达，从而增加非小细胞肺癌细胞的增殖、集落形成以及迁移和侵袭能力。 

LncRNA ABHD11-AS1 promotes proliferation and migration of non-small cell lung cancer cells by regulating STAT1/STAT3 expression

  Objectives   Long-chain non-coding RNA (lncRNA) plays an important role in tumorigenesis and development. At present, lncRNAs are still insufficiently studied in non-small cell lung cancer. This study focused on the biological function and mechanism of lncRNA ABHD11-AS1 in non-small cell lung cancer.  Methods   A total of 248 non-small cell lung cancer patients who underwent surgery in the Affiliated Hospital of Guangdong Medical University from July 2016 to December 2018 were selected. Chi-square test was used to analyze the relationship between the expression of lncRNA ABHD11-AS1 and age, sex, clinical pathological stage, pathological type and smoking status. Kaplan-Meier method was used to analyze the prognostic significance of ABHD11-AS1 in patients with non-small cell lung cancer. qRT-PCR was used to detect the expression of ABHD11-AS1 in non-small cell lung cancer tissues and cell lines. The effect of knockdown of ABHD11-AS1 on cell proliferation and metastasis was evaluated by in vitro functional assay. Western blot was used to investigate the molecular mechanism of ABHD11-AS1 in non-small cell lung cancer.  Results   LncRNA ABHD11-AS1 high expression group was related to advanced stage (P < 0.01) and had fewer smoking patients than lower expression group (P=0.02). ABHD11-AS1 high expression was predictive of poor prognosis in patients with non-small cell lung cancer. ABHD11-AS1 is highly expressed in non-small cell lung cancer tissues and cancer cell lines. Knockdown of ABHD11-AS1 expression can inhibit cell proliferation and metastasis in vitro. Knockdown of ABHD11-AS1 expression inhibits STAT1 and STAT3 oncoproteins expression.  Conclusions   ABHD11-AS1 may promote the proliferation, colony formation, migration and invasion of non-small cell lung cancer cells by promoting the expression of STAT1 and STAT3.