葛根素前体脂质体的制备及体外性质研究

 目的制备葛根素前体脂质体,并对其体外性质进行研究。方法采用载体沉积法制备葛根素前体脂质体,单因素考察和正交实验优选处方;透射电镜观察形态;马尔文激光粒度仪测定粒径、Zeta电位;透析法结合高效液相色谱法测定包封率;动态透析法考察体外释药性质。结果制得的脂质体形态多为圆形或椭圆形,平均粒径为278nm,Zeta电位为-17.5mV,包封率为(43.53±1.33)%,体外释药符合一级动力学方程。结论葛根素前体脂质体包封率较高,具有一定的缓释效果。

Study on Preparation of Puerarin Proliposomes and Its Pharmaceutical Characteristics in vitro

OBJECTIVE To prepare puerarin proliposomes and investigate its pharmaceutical characteristics in vitro.METHODS Proliposomes were prepared by carrier aggradation method.A single-factor investigation and orthogonal design were used to select the optimum formulation. The shape,particle size and Z-potential of puerarin proliposomes were investigated with transmission electron microscope and laser scatter. The encapsulation efficiency was determined by HPLC after liposomes were dialyzed. The release of puerarin from proliposomes was studied in vitro.The appearance and the encapsulation efficiency of proliposomes were used to evaluate the statility of proliposomes. RESULTS The average size of the puerarin liposomes was 278 nm and the Zeta potential was -17.5 mV. The encapsulation efficiency was (43.53±1.33)%. The release in vitro was characterized by the first order equation.The proliposomes was stable when being stored at room temperature. CONCLUSION The encapsulation efficiency of prolipsomes is good . The drug is sustained-released from the liposomes.