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短芽孢杆菌RL-2絮凝机理研究
引用本文:罗平,罗固源,左赵宏.短芽孢杆菌RL-2絮凝机理研究[J].环境工程学报,2007,1(6):39-42.
作者姓名:罗平  罗固源  左赵宏
作者单位:1. 重庆大学化学化工学院,重庆,400044
2. 重庆大学城市建设与环境工程学院,重庆,400045
基金项目:重庆大学校科研和教改项目
摘    要:由编号为RL-2的短芽孢杆菌(Bacillus brevis)产生的生物高分子,经研究发现是一种对高岭土悬液有较高絮凝活性的阴离子型絮凝剂.对絮凝剂的热处理及酶处理表明,其活性成分为多糖;经茚三酮试验,Molish反应,薄层色谱及红外光谱分析表明,该絮凝剂为一种阴离子型多糖絮凝剂;ζ电位测定及絮凝剂与高岭土颗粒之间结合键的检测表明,絮凝剂与高岭土颗粒间的结合力为氢键,絮凝过程基于"桥联"机理;絮凝剂和高岭土在絮凝剂的活性部位--多糖中的羟基或羧基以氢键相结合,经架桥作用絮凝沉淀.

关 键 词:微生物絮凝剂  絮凝机理  架桥作用  短芽孢杆菌  短芽孢杆菌  絮凝沉淀  机理研究  brevis  Bacillus  mechanism  架桥作用  键相  羧基  羟基  活性部位  高岭土  桥联  絮凝过程  氢键  结合力  检测  土颗粒  电位测定  阴离子型
文章编号:1673-9108(2007)06-0039-04
收稿时间:8/8/2006 12:00:00 AM
修稿时间:2006-08-082006-12-05

Study on flocculating mechanism of Bacillus brevis(RL-2)
Luo Ping,Luo Guyuan and Zuo Zhaohong.Study on flocculating mechanism of Bacillus brevis(RL-2)[J].Techniques and Equipment for Environmental Pollution Control,2007,1(6):39-42.
Authors:Luo Ping  Luo Guyuan and Zuo Zhaohong
Affiliation:1. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044 ; 2. Faeuhy of Urban Construction and Environmental Engineering, Chongqing University , Chongqing 400045
Abstract:The biopolymer produced by strain RL-2 was found to be an effective anionic flocculant for Kaolin suspensions. The bioflocculant is treated by heat and enzyme, which indicates that the active ingredient of biofocculant is polysaccharide. RL-2 is mainly composed of anionic polysaccharide, which is determined by Ninhydrin reaction, Molish reaction, TLC and infrared spectrum. From the acting force and zeta-potential measurements, it was concluded that the acting force between bioflocculant and Kaolin suspension is hydrogen bond, the flocculation process is based on the bridging mechanism, the combination of Kaolin suspension with RL-2 by hydrogen bond at the active position of polysaccharide.
Keywords:bioflocculant  flocculating mechanism  bridging  Bacillus brevis
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