Restriction endonuclease analysis of four Borrelia burgdorferi strains |
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Authors: | M Cinco E Banfi D Balanzin S Cacciò G Graziosi P Fattorini |
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Affiliation: | Istituto di Microbiologia, Universitàdi Trieste, Trieste, Italy;Dipartimento di Biologia, Universitàdi Trieste, Trieste, Italy |
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Abstract: | Abstract A restriction endonuclease analysis was performed on four strains of Borrelia burgdorferi : one isolated from man (SF), one from Ixodes dammini (B31) and two form I. ricinus (BITS in Italy and B45 in Germany). Digestion by Taq I and Hae III gave the best resolution of the DNA fragments. Three different restriction patterns were obtained: BITS and B45 showed only one band difference. These results correlate with the reactivity of the four strains with monoclonal antibodies. |
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Keywords: | Borrelia burgdorferi Restriction endonuclease analysis Monoclonal antibody |
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