| 重组乳酸克鲁维酵母产磷脂酶A_2发酵条件的优化 |
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| 引用本文： | 王 辉,张 梁,李由然,顾正华,丁重阳,石贵阳,陈国安,杨盛荣.重组乳酸克鲁维酵母产磷脂酶A_2发酵条件的优化[J].食品科学,2015,36(21):111-116. |
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| 作者姓名： | 王 辉 张 梁 李由然 顾正华 丁重阳 石贵阳 陈国安 杨盛荣 |
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| 作者单位： | 1.江南大学 工业生物技术教育部重点实验室，江苏 无锡 214122；2.江南大学 粮食发酵工艺与技术国家工程实验室，江苏 无锡 214122；3.无锡江大百泰科技有限公司，江苏 无锡 214122 |
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| 摘 要： | 利用重组乳酸克鲁维酵母(Kluyveromyces lactis)GG799表达磷脂酶A2,对其产酶发酵条件进行研究。采用单因素试验和正交试验对培养基及培养条件进行优化,确定了重组菌产酶的最佳发酵条件。结果表明:最优培养基组成为葡萄糖30 g/l、酵母粉20 g/l、蛋白胨30 g/l、Kh2PO4 3 g/l;最优培养条件为:发酵温度30℃、接种量2%(V/V)、初始pH 7.0、装液量90 ml/250 ml三角瓶、摇床转速220 r/min,在此条件下发酵培养,酶活力由(1.87±0.12)U提高到(5.35±0.27)U。
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| 关 键 词： | 乳酸克鲁维酵母 磷脂酶A2 发酵 |
Optimization of Fermentation Conditions for the Production of Phospholipase A2 by Recombinant Kluyveromyces lactis |
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| WANG Hui,ZHANG Liang,LI Youran,GU Zhenghua,DING Zhongyang,SHI Guiyang,CHEN Guoan,YANG Shengrong.Optimization of Fermentation Conditions for the Production of Phospholipase A2 by Recombinant Kluyveromyces lactis[J].Food Science,2015,36(21):111-116. |
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| Authors: | WANG Hui ZHANG Liang LI Youran GU Zhenghua DING Zhongyang SHI Guiyang CHEN Guoan YANG Shengrong |
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| Affiliation: | 1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;
2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;
3. Wuxi Jiangda Baitai Technology Co. Ltd., Wuxi 214122, China |
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| Abstract: | Fermentation conditions for the production of phospholipase A2 (PLA2) by recombinant Kluyveromyces lactis
GG799 were optimized using single factor and orthogonal design experiments. The optimal fermentation medium was
composed of 30 g/L glucose, 20 g/L yeast extract, 30 g/L peptone and 3 g/L KH2PO4. The best fermentation conditions were
determined as 30 ℃,2%, 7.0, 90 mL and 220 r/min for temperature, inoculum amount, initial pH, medium volume in a 250 mL
flask and shaking speed, respectively, resulting in an increase of PLA2 activity from (1.87±0.12) U to (5.35±0.27) U. |
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| Keywords: | Kluyveromyces lactis phospholipase A2 fermentation |
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