| 基于Illumina MiSeq高通量测序技术解析利川酱豆真菌多样性 |
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| 引用本文： | 董蕴,张苗苗,邓子文,剧柠,郭壮,赵慧君.基于Illumina MiSeq高通量测序技术解析利川酱豆真菌多样性[J].中国酿造,2022,41(4):137-141. |
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| 作者姓名： | 董蕴 张苗苗 邓子文 剧柠 郭壮 赵慧君 |
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| 作者单位： | （1.湖北文理学院 湖北省食品配料工程技术研究中心，湖北 襄阳 441053； 2.宁夏大学 食品与葡萄酒学院，宁夏 银川 750021） |
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| 摘 要： | 该研究利用Illumina MiSeq高通量测序技术对湖北省恩施土家族苗族自治州利川市酱豆样品的真菌多样性和群落结构进行解析。结果表明，利川酱豆样品中的真菌菌群隶属于3个门、15个纲、27个目、34个科、45个属，核心真菌门为子囊菌门（Ascomycota）（96.79%）和担子菌门（Basidiomycota）（2.63%）；核心真菌属为毕赤酵母属（Pichia）（37.46%）、曲霉属（Aspergillus）（29.48%）、德巴利氏酵母属（Debaryomyces）（3.92%）、假丝酵母属（Candida）（2.86%）、Starmerella（2.62%）、节担菌属（Wallemia）（2.19%）和镰刀菌属（Fusarium）（1.03%），且核心真菌之间的相关性均不显著（P＞0.05）。
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| 关 键 词： | 利川酱豆 Illumina MiSeq高通量测序技术 真菌多样性 群落结构 |
Analysis of fungal diversity in Lichuan Jiangdou based on Illumina MiSeq high throughput sequencing technology |
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| DONG Yun,ZHANG Miaomiao,DENG Ziwen,JU Ning,GUO Zhuang,ZHAO Huijun.Analysis of fungal diversity in Lichuan Jiangdou based on Illumina MiSeq high throughput sequencing technology[J].China Brewing,2022,41(4):137-141. |
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| Authors: | DONG Yun ZHANG Miaomiao DENG Ziwen JU Ning GUO Zhuang ZHAO Huijun |
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| Affiliation: | (1.Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang 441053, China; 2.College of Food and Wine, Ningxia University, Yinchuan 750021, China) |
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| Abstract: | In this study, the fungal diversity and community structure of Jiangdou samples from Lichuan city, Enshi Tujia and Miao Autonomous Prefecture, Hubei Province were analyzed by Illumina MiSeq high-throughput sequencing technology. The results showed that the fungi in the samples of Lichen Jiangdou belonged to 3 phyla, 15 classes, 27 orders, 34 families and 45 genera. The core fungal phyla were Ascomycota (96.79%) and Basidiomycota (2.63%). The core fungal genera were Pichia (37.46%), Aspergillus (29.48%), Debaryomyces (3.92%), Candida (2.86%), Starmerella (2.62%), Wallemia (2.19%) and Fusarium (1.03%), and there was no significant correlation between core fungi (P＞0.05). |
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| Keywords: | Lichuan Jiangdou Illumina MiSeq high throughput sequencing technology fungi diversity community structure |
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