A versatile multimode microscope to probe and manipulate nanoparticles and biomolecules |
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| Authors: | M KYOUNG K KARUNWI‡ & E D SHEETS† |
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| Affiliation: | Department of Chemistry;and The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park PA 16802, USA;Department of Physics, Georgia Institute of Technology, Atlanta GA 30332, USA |
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| Abstract: | We describe a flexible, multifaceted optical setup that allows quantitative measurement and manipulation of biomolecules and nanoparticles in biomimetic and cellular systems. We have implemented integrated biophotonics techniques (i.e. differential interference contrast, wide‐field fluorescence, prism‐ and objective‐based total internal reflection excitation, single particle tracking, fluorescence correlation spectroscopy and dynamic holographic optical trapping) on a single platform. The adaptability of this versatile, custom‐designed system allows us to simultaneously monitor cell morphology, while measuring lateral diffusion of biomolecules or controlling their cellular location or interaction partners. |
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| Keywords: | Fluorescence correlation spectroscopy fluorescence microscopy holographic optical trap single particle tracking total internal reflection fluorescence |
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